利用高效液相層析法建立雞肉、雞肝、牛肉及牛肝中拉薩羅(lasalocid)殘留量之分析方法。拉薩羅以乙　自檢體中萃取，再加入正己烷去雜質及濃縮後，經矽膠層析匣（silica Sep-Pak cartridge）淨化，最後利用高效液相層析儀以螢光檢測分析。層析管為Cosmosil5C18-AR-Ⅱ (5μm, 4.6×150mm)，移動相為甲醇/0.02M KH　PO　(84/16)，於激發波長310 nm及放射波長420 nm偵測。檢體中添加拉薩羅0.05、0.1 及0.2 ppm時，其回收率分別為雞肉93.17~95.61%，雞肝91.53~93.59%，牛肉96.93~97.70%及牛肝 90.42~95.92%，變異係數皆小於5%。本方法在四種檢體中之最低檢出限量均為0.005ppm。利用此方法分析市售雞肉、雞肝、牛肉及牛肝檢體共20件，結果均未檢出。目前我國規定肉品中不得殘留拉薩羅。

A reverse phase high performance liquid chromatographic (RP-HPLC) method for the determination of lasalocid in the muscle and liver of chicken and cattle was developed. Lasalocid was extracted form samples with acetonitrile. After filtration, the extract was purified by liquid-liquid partition, concentrated by rotary evaporation, purified with a silica Sep-Pak cartridge, and finally determined by fluorimetric HPLC. The separation was performed on a Cosmosil 5C18-AR-Ⅱcolumn (5μm, 4.6×150mm) with methanol/0.02M KH2PO4(84/16, v/v) as the mobile phase, and lasalocid was detected at Ex 310 nm and Em 420 nm. Recovery studies were performed at the 0.05,0.1 and 0.2 ppm fortification levels in each of the four commodities. Average recoveries were in therange of 93.17~95.61%, 91.53~93.59%, 96.93~97.70% and 90.42~95.92% from chicken muscleand liver, and bovine muscle and liver, respectively. Coefficients of variation for average recoverieswere less than 5%. The limit of detection was 0.005 ppm in these four commodities. When 20samples purchased from various markets in Taipei were analyzed, no lasalocid was detected.ed.