本研究利用高效液相層析儀，分析番瀉葉、番瀉莢果及市售減脂茶中sennoside A及B之含量，採用C 18逆相層析管柱，移動相為乙腈0.1％醋酸溶液，利用線性梯度沖提，檢測波長為270nm；得到良好分析結果。Sennoside A及B二成分之線性迴歸方程式及相關係數（r）分別為： sennoside A（濃度 7.52～37.60 μg/ml），Y=32.07032X-0.1098656（r=0.9998）； sennoside B（濃度 8.32 ～ 49.92 μg/ml），Y=28.13735X+0.2604257（r=0.9999），均呈現良好線性關係。添加回收率試驗結果，番瀉葉之sennoside A為100.7±0.90％，sennoside B 為102.3±1.31％；番瀉莢果之 sennoside A為106.6±0.50％，sennoside B為102.5±2.17％，顯示準確性佳。同日內及異日間試驗之相對標準偏差，分別為0.24～1.72％及0.23～1.71％，顯示高效液相層析法用於sennoside A及B之分析其結果良好。
利用上述高效液相層析法定量番瀉葉及番瀉莢果內sennoside A及B的含量，結果番瀉葉中的 sennoside A及B含量分別為0.64、1.57％，番瀉莢果中的 sennoside A及B的含量分別為1.46、2.30 ％。依此法分析不同七種市售減脂茶中 sennoside A 及 B 的含量，結果sennoside A之含量則在 0.10～0.19％之間；sennoside B之含量在 0.16～0.28％之間。

　　　　　A rapid high performance liquid chromatographic method was developedfor determination of sennosides A and B in senna leaves, pods and diet tea. Thedetermination was carried out by using a gradient elution with acetonitrile and1% acetic acid on an Inertsil ODS-2 column.The linear cablibration ranges of sennosides A and B were 7.52 ～ 37.60 μg/ml (r=0.9998) and 8.32 ～ 49.92 μ g/ml (r=0.9999), respectively. Therecoveries of sennosides A and B were almost quantitative from the senna leavesand pods, respectively. The relative standard deviations of sennosides A and Bfor intraday and interday analyses were 0.24 ～ 1.72% and 0.23 ～ 1.71%,respectively. The contents of sennosides A and B in leaves and pods of Cassiaangustifolia Vahl have been determined and the results were as follows:sennoside A 0.64, 1.46% and sennoside B 1.57, 2.30%. The contents of sennosidesA and B in seven diet tea samples have been determined and the results were asfollows: sennoside A 0.10 ～ 0.19% and sennoside B 0.16 ～ 0.28%.