本研究利用毛細管電泳儀，分析玄參藥材中之高極性成分2-(3-hydroxy-4-methoxyphenyl) ethyl 1-O-[α-L-arabinopyranosyl(1→6)]-feruloyl (1→4)-α-L-rhamnopyranosyl (1 → 3)-β-D-glucopyranoside (SN-A), harpagoside (SN-B)及 cinnamic acid (SN-C) 含量，以 20mM sodium tetraborate 及 sodium dihydrogenphosphate 調配為 pH 7.5 含 100mM sodium cholate 及10 ％乙腈之溶液為緩衝液，內部標準品為 propylparaben，檢測波長為280nm，得到良好的分析結果。SN-A、SN-B 及 SN-C三成分之相關係數（ｒ）分別為：SN-A濃度20.0～320.0 μg/ml,（r=0.9994）；SN-B濃度 9.6～153.6 μg/ml,（r=0.9994）及SN-C濃度1.6～25.6 μg/ml），（r=0.9996），均呈現良好線性關係。添加回收率試驗結果，SN-A 為 103.5 ± 0.4％，SN-B 為 102.4 ± 2.9 ％，SN-C 為 102.4 ± 1.9 ％。三種指標成分之同日間及異日間試驗之相對標準差，分別為 0.55～1.71 ％及 1.36～3.83 ％，顯示再現性佳。本研究並探討分析條件中緩衝液之pH值、界面活性劑之濃度、溫度、電壓及修飾劑，對上述成分移動時間之影響。

A rapid method for simultaneous determination of three marker constituents, 2-(3-hydroxy-4-methoxyphenyl) ethyl 1-O-[α-L-arabinopyranosyl(1→6)]-feruloyl(1→4)-α-L-rhamnopyranosyl(1→3)-β-D-glucopyranoside(SN-A), harpagoside (SN-B) and cinnamic acid (SN-C) in Scrophulariae Radix by micellar electrokinetic capillary chromatography was developed. In this study, the effects of analytical conditions, including buffer pH, buffer electrolyte, temperature, applied voltage and organic modifier concentration, on separations were studied. The optimal chromatographic conditions were obtained with a buffer composed of 20 mM sodium tetraborate and sodium dihydrogen phosphate containing 100 mM sodium cholate and 10% acetonitrile at pH 7.5. Propylparaben was used as an internal standard and analytes were detected at 280 nm. The linear calibration range of SN-A, SN-B and SN-C were 20.0-320.0 μg/ml (r=0.9994), 9.6-153.6 μg/ml (r=0.9994) and 1.6-25.6 μg/ml (r=0.9996), respectively. The recoveries of these markers were SN-A:103.5±0.4%, SN-B:102.4±2.9% and SN-C:102.4±1.9%. The relative standard deviations of the three marker substances for intraday and interday analyses were 0.55-1.71% and 1.36-3.83%, respectively.