目的:芳香胺化合物經N基乙醯轉移酵素之N基乙醯化後可誘發癌□症，本研究是in vitro探討大白鼠神經膠質培養腫瘤細胞中2-AF-DNA的形成。材料與方法:取大白鼠神經膠質培養腫瘤細胞利用乙醯化輔□A作乙醯基及γ-[32p]-dATP而再利用高壓層析儀分析2-AF-DNA的形成性，以aminofluorene(AF)作受質。結果:結果大白鼠神經膠質培養腫瘤細胞有乙醯轉移酵素的活性存左，其對濃度30 uM and 60uM AF為受質形成2-AF-DNA adducts各為0.48±0.16 and 0.70±0.12 pmol/mg DNA。結論:結果發現大白鼠神經膠質培養腫瘤細胞中乙醯轉移酵素，可將2-AF乙醯化成2-AAF與細胞中去氧核糖核酸□結合為2-AF-DNA。本研究報告是in vitro探討的初步報告,未來需進一步以in vivo的實驗確認。

　　　　　Purpose: Arylamine N-acetylation capacity by the N-acetyltransferase (NAT) may be animportant causative factor in the initiation of cancer. Most carcinogens have been demonstrated toreact with cellular DNA to form covalent DNA adducts. Arylamine-DNA adducts formation havebeen correlated with the carcinogenic effect of heterocyclic aromatic amines. 2-AF-DNA formationin rat glial cultured tumor cell line was investigated.Materials and Methods: 2-aminofluorene-DNA (2-AF-DNA) adducts formation in rat glial culturedtumor cell line was investigated by γ-[32p]-dATP and HPLC, using 2-aminofluorene as substrates.Results: 2-AF-DNA adducts formation in rat glial cultured tumor cell with 30 uM and 60 uM AFwere 0.48±0.16 and 0.70±0.12 pmol/mg DNA, respectively.Conclusion: The results indicate that rat glial cultured tumor cells activate AF to a metabolite ableto bind covalently with DNA, and also induced dose-dependent effect.