將76株分離自動物及其產製品之金黃色葡萄球菌(生乳62株、鯨魚3株、海豚4株、乳房炎牛乳汁 2 株、豬 2 株，雞、鼠、犬各 1 株 )，以商品化之 ELISA 套組檢測其腸毒素產生能力， 結果有 26 株產生 A 型腸毒素，產生 B、C 型腸毒素者分別有 2 株及 1 株，而未檢出產生 D、E 型腸毒素之菌株。另以自行設計之引子對 (primers)，以毛細管進行聚合酶鏈反應 (polymerase chain reaction， PCR) 檢測帶有產生 A 型腸毒素基因之菌株，結果亦有 26 株呈陽性反應， 與 ELISA 之檢測結果相同，但 PCR 檢測 A 型腸毒素基因之靈敏度為10 4 CFU/ml。

A commercial ELISA kit (RIDASCREEN R SET A, B, C, D, E. R-Biopharm GmbH, Darmstadt, Germany) was used to examine the enterotoxigenecity of 76 Staphylococcus aureus strains. Of these strains, 62 were isolated from raw milk, 3 from whale, 4 from dolphin, 2 from cow milk with mastitis, 2 from pig, and 1 each from chicken, mouse, and dog. Type A, B, and C enterotoxins were detected in 26, 2, and 1 strains, respectively. Neither type D nor type E enterotoxin-producing strain was observed. In addition, DNA amplification by capillary polymerase chain reaction (PCR) with specific primers was used to detect the presence of enterotoxin A gene. The results showed that 26 strains harbored this gene. Study on the detection sensitivity of PCR indicated that the lowest cell number required for positive reaction was 10 4 CFU/ml.