蛋白質的立體結構是近幾年用來研究生物功能的熱門主題，X光晶體繞射與核磁共振為此領域中相當重要的方法。國立清華大學生命科學院結構生物實驗室在國家科學委員會基因體醫學國家型科畫的支持下，進行胃幽門螺旋桿菌結構基因體之研究。在本文中，將介紹這兩種方法的原因理及如格應用在胃幽門螺旋桿菌之結構基因體的研究。蛋白質必須具有正確的摺，才能執行它應有的生物誤。因此可以藉著研蛋白質立體結構，來了解此蛋白質的生物誤。同時可以精確地決定蛋白質的功能區域以及關鍵的胺基酸，此方法可達到高於奈米的原子解析度。

The three-dimensional structure of protein is an essential topic for the biochemical function research in recent year. X-ray diffraction and nuclear magnetic resonance (NMR) are two important methods in this field. In this article, we will introduce the principles and circumstance of these two methods, and how we apply these two methods in our study: Helicbacter pylori structural genomics research. Proteins carry out their biological functions only when they fold correctly. A unique protein folding respects to have a specific biological function. So, we can investigate the three-dimensional structure of protein to study the possible biological function of protein. We determine the active site of protein and the key residues involved in the biological function. The resolution of these methods is in atomic resolution.