篇名 | Effect of Different Concentrations of Collagen, Ceramides, N-acetyl glucosamine, or Their Mixture on Enhancing the Proliferation of Keratinocytes, Fibroblasts and the Secretion of Collagen and/or the Expression of mRNA of Type I Collagen |
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卷期 | 16:1 |
作者 | RONG-HUEI CHEN 、 CHI-NAN HSU 、 MEI-YUH CHUNG 、 WEN-LIN TSAI 、 CHIA-HAN LIU |
頁次 | 66-74 |
關鍵字 | collagen 、 N-acetyl glucosamine 、 ceramides 、 mixture 、 proliferation 、 keratinocytes 、 fibroblasts 、 mRNA 、 Type I Collagen 、 expression 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200802 |
The objectives of the study are to survey the effects of different concentrations of collagen, ceramides, N-acetyl glucosamine (NAG), or their mixture on the proliferation of keratinocytes, fibroblasts and on the secretion of collagen and/or the expression of mRNA of type I collagen. The optimal concentrations that facilitated the proliferation of fibroblasts were 49, 2.0, and 24.4 μg/mL, respectively for collagen, ceramides, and NAG. The proliferation percentages were 191%, 170%, and 223%, respectively at the same order in compare to the control. The concentration of the mixture containing 6.1, 0.12 and 6.1 μg/mL, respectively of collagen, ceramides, and NAG facilitated the proliferation of fibroblasts. The optimal concentrations that had highest keratinocytes proliferation were 0.76, 0.001, and 0.76 μg/mL, respectively for collagen, ceramides, and NAG. The proliferation percentages were 242%, 203% and 230%, respectively at the same order in compare to the control. The optimal concentration that effects the highest keratinocytes proliferation by the mixture were 0.38, 0.008 and 0.38 μg/mL for collagen, ceramides and NAG, respectively. The concentrations for fibroblasts to express higher amount of type I collagen mRNA were 6.1, 0.49, and 6.1 μg/mL, respectively for collagen, ceramides, and NAG. The expression levels of type I collagen mRNA were 152%, 160% and 220%, respectively at the same order in compare to the control. However, the expressions of type I collagen mRNA were 180% in compare to the control at the concentration of mixture containing 0.76, 0.015 and 0.76 μg/mL, respectively of collagen, ceramides, and NAG.