經過數十年在實驗技術與硬體設備的進步與發展,核磁共振(NMR)光譜學已成為研究生物巨分子不可或缺的重要工具之一。本文旨在簡介最近在實驗技術上的重要進展,如用以提升巨大生物分子(>50kDa)的光譜靈敏度與解析度,及蛋白質結構優化等技術。除此之外,傳統多維NMR實驗是非常冗長而耗時的,此為核磁共振相關技術最大的缺點,故本文亦簡介最近在快速多維核磁共振實驗技術方面的發展。
Nuclear magnetic resonance (NMR) spectroscopy, extensively and successfully employed in many fields, has become one of the most important tools for studying biological macromolecules with the remarkable progress in both experimental techniques and hardware improvement in the past decades. This article briefly introduces recent significant progress in experimental techniques capable of improving sensitivity and resolution of spectra for very large biomolecules (> 50 kDa) and refinement of protein structures. In addition, conventional experiments of multidimensional NMR are very time-consuming to achieve well-resolved spectra. Recent advance of experiment techniques for shortening days-long acquisition time is also mentioned.