The aim of this study was to develop an easy analytical method for determining aldicarb, propoxur, carbofuran, carbaryl and methiocarb residues in honey by high performance liquid chromatography with post-column derivatization and fluorescence detection (HPLC/PCD-FL) for routine analysis. A florisil packed column was used in sample preparation step and showed good performance. The recoveries ranged between 72.02 and 92.02% for various spiking levels (50, 100, 200 ng/g). The limits of detection were 4–5 ng/g, depending on different N-methylcarbamate pesticides. The extraction method used in the present study was easy, inexpensive, and rapid. Thus, it could be used efficiently in the routine monitoring studies.