人類上皮生長因子(hEGF)為53個胺基酸殘基所組成的單鏈蛋白質，其分子量約為6.2 KDa。hEGF可刺激各種細胞的生長和抑制人類胃酸的分泌，可能被廣泛運用於傷口癒合、角膜移植和治療胃潰瘍。為了獲得足夠量的hEGF。本實驗利用重組DNA技術將hEGF基因接入pQE31表現載體，再經由PCR技術、SDS-PAGE電泳分析和西方轉漬法進行確認，成功的建構可於大腸桿菌JM109宿主細胞內表現hEGF蛋白的重組質體，命名為pGF3101。hEGF生產條件，IPTG誘導物濃度為1mM，誘導時間為5小時，達到較良好的表現程度。此外，在hEGF蛋白的N端接上一段6個His，有利於純化過程的簡化。

Human epidermal growth factor (hEGF), consisting of 53 amino acid residues, was a single chain polypeptide with a molecular weight of about 6,2 KDa. hEGF was widely used in wound healing, corneal transplants, and the treatment of gastric ulcers. In order to obtain hEGF in sufficient quantities, recombinant DNA technology is extensively used. The pGF3101, harboring the synthetic hEGF gene in the expression vector pQE31, is expressed in Escherichia coli JM109 and the protein expression are identified by PCR technology, SDS-PAGE, and Western blotting. The condition of hEGF expression was by adding IPTG to a final concentration of 1 mM and the induction time was for an additional 5 hours. Furthermore, it will be easy to purify the hEGF protein with N-terminal 6xHis affinity tags.