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藥物食品分析 MEDLINESCIEScopus

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篇名 利用毛細管電泳鑑別燕窩
卷期 6:1
並列篇名 Application of Capillary Electrophoresis for Identification of the Authenticity of Bird's Nests
作者 蘇淑珠余珮菁劉兆宏蕭惠文李樹其周薰修
頁次 455-464
關鍵字 燕窩鑑別毛細管電泳胺基酸Bird's nestIdentificationCapillary electrophoresisCEAmino acidsMEDLINEScopusSCIE
出刊日期 199803

中文摘要

     利用毛細管電泳分析胺基酸組成鑑別燕窩真偽,燕窩檢體經酸及鹼水解後,以dansyl chloride 衍生化, 再以毛細管電泳分析。 胺基酸之回收率(包括 NH ?隻b內)為102.30 %,檢出限量為 0.01 ~ 0.03 pmole。另就全省各中藥店及超級市場所購得之十件燕窩藥材及二十件冰糖燕窩製品,進行分析。燕窩藥材之粗蛋白質為 47.05 ~ 58.91 %。冰糖燕窩製品之固形物含量為 2.29 ~ 54.45 %, 粗蛋白質為 1.44 ~ 58.45 %。 於UV 365 nm 下,燕窩藥材多半呈淡藍白色螢光,冰糖燕窩製品較難判定。由胺基酸分析圖譜發現燕窩真品具相同之胺基酸 profile,而冰糖燕窩製品中,一件疑為白木耳及其他物質之混合加工品,五件因未檢出胺基酸可能是以洋菜、燕石或海藻酸鈉製成之替代品,另有一件檢出 hydroxyproline 疑似以豬皮加工製成。

英文摘要

     A method using capillary electrophoresis (CE) to determine amino acidprfile was developed to identify the authenticity of bird's nest. Bird's nestsamples were hydrolyzed in acid and alkali, respectively. The proteinhydrolysates were then derivatized with dansyl chloride, and analyzed by CE. Theaverage recovery of amino acids was 102.30%. The detection limits were between0.01 and 0.03 pmole.Ten bird's nest samples and twenty bird's nest in rock sugar syrup wereanalyzed. The bird's nest samples were found to contain 47.05 ~ 58.91% crudeprotein, while bird's nest in rock sugar syrup were found to contain 1.44 ~58.45%. All the bird's nest samples were identified to be authentic based onamino acid profile matching. Among 20 bird's nest in rock sugar syrup, sevenwere considered to be substituted with agar, natural gum or pork skin. CEanalysis of amino acid profile was found to be an efficient and sensitivetechnique for identifying the authenticity of bird's nests.

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