絲瓜果實經刀片輕切裂口，塗上含抗氧化劑的萃取液後，其與韌皮部溢流物一起收集。以混合液經活性與變性(SDS)的兩次電泳分析。一堆分離獲得85kDa活性蛋白質，其約佔全蛋白質的28.2%，其在二維電泳膠上呈五條帶，其中的42.5kDa的變性蛋白質在高效能電泳色層分析顯示單一峰帶，而在變性銀染色膠上僅呈一蛋白質帶。

　　　　　Phloem exudates leaking from Luffa cylindrica fi-uits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimensional gel, i. e., native and sodium dodecyisulfate (SDS) polyacrylamide gel electrophoresis (PAGE). A major protein of 85 kDa occupying 28.2% of total protein had 5 bands on denatured gel. One of them with molecular weight at 42.5 kDa was complementally identified by reversed-phase high performance liquid chromatography and silver stained-band on SDS-gel.