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篇名 Isolation and Characterization of New Sporamin Gene Members from Sweet Potato (Ipomoea Batatas Lam.)
卷期 42:1、42:1
並列篇名 甘藷中Sporamin基因群的選殖及特性研究
作者 陳仁治陳益明葉開溫
頁次 34-42
關鍵字 Multigene familySporaminStorage proteinSweet potato3'-untranslation region 多基因型Sporamin基因群貯藏性蛋白質甘薯3'-尾終端不轉譯區ScopusTSCISCIE
出刊日期 199703

中文摘要

     本文中報告了四個Sporamin cDNA基因,其中有二個是全長的基因,另二個則不完整;此四個cDNA基因其序列與以往發表的皆不盡相同,但相似度則達90% 以上;根據Gene Bank 的資料分析,四者皆屬Sporamin A亞群分子;從南方雜合的資料顯示,其含有相當多的子基因,北方轉印資料顯示,此基因在葉子部不表現,在莖部則微量存在,然而在塊根中則大量表現,故此基因被推論為扮演甘藷塊根中貯藏性蛋白質的功能。

英文摘要

     Two full-length and two partial cDNA s encoding sporamin A have been isolated from sweet potato tuberous roots. Sequence comparisons show that they are very similar with 94-98% homology at nucleotide level, and 80-88% at protein level. All four cDNAs possess multiple alternate polyadenylation signals in the 3' untraslated region (3'-UTR). Genomic Southern blot analysis indicates the presence of a sporamin multigene family in sweet potato. High levels of sporamin mRNAs were detected in developing tuberous roots, but they disappeared at the sprout-germinating stage. Differential expression of these genes was obvious as their mRNAs were present specifically in developing roots, rarely in stems and not in levaves.

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