篇名 | Isolation and Characterization of New Sporamin Gene Members from Sweet Potato (Ipomoea Batatas Lam.) |
---|---|
卷期 | 42:1、42:1 |
並列篇名 | 甘藷中Sporamin基因群的選殖及特性研究 |
作者 | 陳仁治 、 陳益明 、 葉開溫 |
頁次 | 34-42 |
關鍵字 | Multigene family 、 Sporamin 、 Storage protein 、 Sweet potato 、 3'-untranslation region 、 多基因型 、 Sporamin基因群 、 貯藏性蛋白質 、 甘薯 、 3'-尾終端不轉譯區 、 Scopus 、 TSCI 、 SCIE |
出刊日期 | 199703 |
本文中報告了四個Sporamin cDNA基因,其中有二個是全長的基因,另二個則不完整;此四個cDNA基因其序列與以往發表的皆不盡相同,但相似度則達90% 以上;根據Gene Bank 的資料分析,四者皆屬Sporamin A亞群分子;從南方雜合的資料顯示,其含有相當多的子基因,北方轉印資料顯示,此基因在葉子部不表現,在莖部則微量存在,然而在塊根中則大量表現,故此基因被推論為扮演甘藷塊根中貯藏性蛋白質的功能。
Two full-length and two partial cDNA s encoding sporamin A have been isolated from sweet potato tuberous roots. Sequence comparisons show that they are very similar with 94-98% homology at nucleotide level, and 80-88% at protein level. All four cDNAs possess multiple alternate polyadenylation signals in the 3' untraslated region (3'-UTR). Genomic Southern blot analysis indicates the presence of a sporamin multigene family in sweet potato. High levels of sporamin mRNAs were detected in developing tuberous roots, but they disappeared at the sprout-germinating stage. Differential expression of these genes was obvious as their mRNAs were present specifically in developing roots, rarely in stems and not in levaves.