篇名 | Cloning and Characterization of Rice Manganese Superoxide Dismutases |
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卷期 | 42:1、42:1 |
並列篇名 | 水稻含錳超氧歧化?之選殖與性質研究 |
作者 | 陳志成 、 魏道行 、 潘素美 |
頁次 | 53-62 |
關鍵字 | Mn-SOD cDNA 、 Oryza sativa 、 SOD 、 Superoxide dismutase 、 基因選殖 、 水稻 、 超氧歧化? 、 Scopus 、 TSCI 、 SCIE |
出刊日期 | 199703 |
水稻含錳超氧歧化?t於聚丙烯醯胺電泳分離後該酵素素活性具多型性。從水稻 cDNA 庫篩選得到兩個含錳超氧歧化?t之全長cDNA(951,1352 bp),其彼此間之全長和碼定蛋白質對應序列,分別具85.27%和98.71% 相似;而比較二者5'UTR和3'UTR序列,各有59.29%和85.92%相似。南方轉印結果顯示水稻含錳超氧歧化?t為多基因族。
There are multiple forms of manganese superoxide dismutase (Mn-SOD) in rice extract, which can be separated by native gel electrophoresis. We have isolated two rice cDNA clones encoding Mn-SOD designed as msod1 and msod2. The two clones contain 951 and 1352 bps with sequence identity of 85.27% in full length and 98.71% in the coding region. However, the 5' regions prior to the first in-frame start codon (113bp in msod1 and 511bp in msod2) are only 59.29% identical, but the 3' untranslated regions (142bp in msod1 and 145bp in msod2) share 85.92% similarity. Genomic Southern analysis indicates that multiple genes encoding Mn-SOD in rice are multiple in nature.