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篇名 Cloning and Characterization of Rice Manganese Superoxide Dismutases
卷期 42:1、42:1
並列篇名 水稻含錳超氧歧化?之選殖與性質研究
作者 陳志成魏道行潘素美
頁次 53-62
關鍵字 Mn-SOD cDNAOryza sativaSODSuperoxide dismutase基因選殖水稻超氧歧化?ScopusTSCISCIE
出刊日期 199703

中文摘要

     水稻含錳超氧歧化?t於聚丙烯醯胺電泳分離後該酵素素活性具多型性。從水稻 cDNA 庫篩選得到兩個含錳超氧歧化?t之全長cDNA(951,1352 bp),其彼此間之全長和碼定蛋白質對應序列,分別具85.27%和98.71% 相似;而比較二者5'UTR和3'UTR序列,各有59.29%和85.92%相似。南方轉印結果顯示水稻含錳超氧歧化?t為多基因族。

英文摘要

     There are multiple forms of manganese superoxide dismutase (Mn-SOD) in rice extract, which can be separated by native gel electrophoresis. We have isolated two rice cDNA clones encoding Mn-SOD designed as msod1 and msod2. The two clones contain 951 and 1352 bps with sequence identity of 85.27% in full length and 98.71% in the coding region. However, the 5' regions prior to the first in-frame start codon (113bp in msod1 and 511bp in msod2) are only 59.29% identical, but the 3' untranslated regions (142bp in msod1 and 145bp in msod2) share 85.92% similarity. Genomic Southern analysis indicates that multiple genes encoding Mn-SOD in rice are multiple in nature.

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