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篇名 Dynamics of Ultrastructural Characters of Drosophyllum Lusitanicum Link (Droseraceae) Digestive Glands during Maturation and after Stimulation
卷期 50:3
並列篇名 露葉毛氈苔之消化性分泌毛茸在成熟過程與受刺激後微細構造的動態變化
作者 Vassilyev, Andrey E.
頁次 167-182
關鍵字 Acid hydrolase secretionCarnivorous plantsCoated vesiclesDrosophyllumMorphometryUltrastructure酸性水解?之分泌食蟲植物被膜小囊胞形態計量微細構造ScopusTSCISCIE
出刊日期 200509

中文摘要

  本研究針對露葉毛氈苔Drosophyllum lusitanicum Link(Droseraceae)分泌酸性水解?之消化性毛茸的分泌細胞的內膜系統加以量化的探討。分泌毛茸之細胞的粗內質網膜系表面的密度,以及高基氏體、clathrin被膜小囊胞(CCVs)與其衍生的平滑小囊的胞數,均隨著分泌毛茸的成熟而逐漸增加,在完全成熟而未刺激之毛茸的每一分泌細胞,含有之粗內質網膜系的平均表面積為17500μm2以及16000個小胞(包括平均直徑為81 nm的CCVs與其衍生成之平均直徑為46 nm的小囊胞)。在捕捉模擬餌食之刺激後一小時,粗內質網膜系表面的密度、高基氏體的個數、以及蛋白質小囊胞均大量增加,實驗數據顯示由CCVs衍生之平滑小囊胞的形成即意味著分解?的入泌。分泌毛茸在未成熟時即開始分泌水解?,且在成熟時期不論其是否久已捕捉到昆蟲均繼續分泌。藉由刺激,消化性毛茸合成的水解?與其分泌的速率均明顯提高,並造成葉面上消化性液體的釋出。除了蛋白質小囊胞,尚會產生其化兩類之較大的高基氏體分泌小囊胞;顆粒纖纖狀者(直徑大約105 nm,其侷限在分生時期的細胞)以及疏鬆纖維狀者(直徑大約250 nm,僅形成於細胞壁向內生成堆積的時期)。本文提出模式以說明消化性毛茸分泌細胞在刺激之前與刺激之後的功能。

英文摘要

  A quantitative investigation was conducted on the endomembrane system of the secretory cells of Drosophylum lusitanicum Link digestive gladns exporting acid hydrolases. The suface density of rough endoplasmic reticulum (RER) membranes as well as the number of Golgi stacks, clathrin-coated vesicles (CCVs) and their smooth derivatives increased progressively with the gland maturation. On aveage secretory cells of the fully mature unstimulated gland, each contained RER with a suface area of 17500μm2 and 16000 small vesicles (including CCVs of 81 nm aveage diameter and smooth vesicles as the derivatives of CCVs which average 46 nm). One hour after stimulation imitating prey capture, the suface density of RER, the number of Golgi stacks and “protein” vesicles all increased greatly. The data obtained indicate that smooth vesicular derivatives of CCVs are involved in the secretion of digestive enzymes. Hydrolase secretion stared in the immature glands and continued in the mature stage irrespective of whether or not an insect was actually captured. Stimulation resulting in the release of digestive fluid on the surface of the leaf by the digestive galdns appeared to be accompanied by a significant increase in the rate of hydrolase synthesis and secretion. In addition to protein vesicles, two other types of larger secretory Golgi vesicles were produced: granular-fibrillar ones (~105 nm in diameter that were confined to cells at the meristem stage), and loosely-fibrillar vesicles (~250 nm in diameter that occurred only at the stage of wall ingrowth deposition). Models are proposed that depict digestive gland cell functioning both before and after simulation.

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