以Vibrio anguillarum和Vibrio alginolyticus作為測試菌株。利用限制□HindIII和EcoRI將細菌的基因體DNA切割後,選出介於0.5-2.0 kb之片段選殖到質體pUC19上。利用非放射性探針標定篩選的片段,由102選殖的片段中找到一段只能與V. alginolyticus雜交;由94選殖的片段中找到三段只能與V. anguillarum雜交的片段,並找到一段只能與V. anguillarum血清型C作用的片段。

　　　　　Vibrio anguillarum and Vibrio alginolyticus were used as tested organisms. Restrictionendonuclease HindIII and EcoRI digested DNA fragments from the above two bacteria wererandomly selected and inserted into vector pUC19. Among 0.5-2.0 kb DNA fragments wererecovered from agarose gel to prepare non-radioactive DIG-labeled probes. One out of 102 clonedfragments could hybridize only to V. alginolyticus. Three out of 94 cloned fragments couldhybridize only to V. anguillarum and one fragment is specific for serotype C.