葡萄?轉運子4號(glucose transporter 4, GLUT4)為人體骨骼肌肉葡萄??用重要因子之一，文獻顯示?鼠及人體骨骼肌肉GLUT4 mRNA及蛋白質表現?受到運動或碳水化合物補充影響。這次實驗目的想要?解單一運動後?即補充?同升?指?(glycemic index, GI)碳水化合物對骨骼肌肉GLUT4蛋白質表現的影響。24名受試者（?齡21.30±1.46歲，身高173.10 ± 5.33公分，體重70.21 ± 7.34公斤，相對攝氧?峰值43.65±10.58毫升∕公斤∕分鐘），單一次75%最大攝氧?峰值腳踏?運動60分鐘後，隨機分成3組，分別給予禁食、高GI碳水化合物及低GI碳水化合物三種?同試驗處?（禁食組、高GI組、低GI組）。運動剛結束及運動後3小時各進?一次肌肉穿?取得肌肉樣本，以西方墨點法(Western Blotting)定?葡萄?轉運子蛋白質表現，運動剛結束至3小時內，每30分鐘收集血液樣本後，測定血液葡萄?及胰島素的濃?。結果顯示高GI碳水化合物補充組胰島素曲線下面積(insulin area under curve, IAUC)大於低GI碳水化合物補充組(p<.05)，運動後3小時高GI組GLUT4蛋白質表現顯著高於低GI組及禁食組(p<.05)。這次研究結果主要發現，運動後恢?期補充高GI碳水化合物飲食能增加胰島素的分?，並促進GLUT4蛋白質表現。

Glucose transporter subtype 4 (GLUT4) is an important factor for glucose uptake in human skeletal muscle. The mRNA and protein level of GLUT4 can be regulated with exercise or carbohydrate supplementation in rat and human skeletal muscles. This study investigated the effect of different glycemic index (GI) carbohydrate supplementations after a bout of exercise on GLUT4 protein expression in human skeletal muscle. Twenty-four subjects (age 21.30±1.46 yrs, height 173.10±5.33cm, weight 70.21±7.34 kg, VO2 peak 43.65±10.58ml/kg/min) were asked to take a single bout of cycle ergometer at 75% peak oxygen consumption for 60 minutes. The subjects were randomly assigned to one of the three treatment groups after exercise: fasting, high GI carbohydrate (HGI) supplement or low GI carbohydrate (LGI) supplement. Biopsies were performed on the deep portion of the vastus lateralis muscle of all the subjects immediately after exercise and 3 hours after the carbohydrate ingestion. The protein expressions of GLUT4 were shown quantitatively with the Western Blotting technique. The blood samples were collected immediately after the exercise and every 30 minutes thereafter, up to 3 hours. The blood samples were collected for glucose and insulin analyses. Insulin AUC was significantly higher in the HGI group than in the LGI group (p＜.05). The GLUT4 protein content in the HGI group significantly increased above the LGI group and the fasting trail (p＜.05). The results of this study found that the HGI carbohydrate supplement after exercise can enhance GLUT4 protein expression and increase insulin secretion during the recovery period.