目的：在罹患惡性黑色素瘤的病患中，轉型生長因子β被視為降低病患對抗腫瘤免疫反應的重要因子，本研究之目的是要以核糖核酸干擾抑制技術降低黑色素瘤細胞內轉型生長因子β1的表現，藉此達成抑制黑色素瘤在小黑鼠皮下的生長。材料與方怯：我們將設計好的轉型生長因子β1寡核?酸構築到一個反轉錄病毒載體pSM2，將構築成功的質體命名為TGF-β1-RNAi/pSM2並穩定轉植到小黑鼠本研究主要目的是探討大學生知覺體育教師?導?為影響運動健康信?、身體活動?為與意圖的因果模式。本研究以485名大專校院學生為研究對象（男237名，?248名）。本研究所用工具為體育教師?導?為?表、運動健康信??表、身體活動?為問卷及身體活動意圖?尺。所得資?以徑?分析加以驗證。研究結果如下：一、訓?與教學的?為、管?的?為可直接影響知覺運動??；讚賞的?為、溝通的?為可直接影響?動線?。二、知覺運動障礙與?動線?可直接影響身體活動?為。三、知覺運動障礙與知覺運動??可直接影響身體活動意圖。本研究結果可供大學體育教師做為教學上的?考，以提升學生的運動健康信?，?進而影響其運動的意圖和身體活動?為。黑色素瘤細胞，B16F0細胞株，將轉植質體後的細胞命名為B16F0/TGF-β1-RNAi細胞，同時也將反轉錄病毒載體pSM2穩定轉植到小黑鼠黑色素瘤細胞並命名為B16F0/vector-control細胞，作為實驗中的載體控制組細胞，之後分別將5×10^6個野生型腫瘤細胞(B16F0)、載體控制組細胞(B16F0/vector-control)或是B16F0/TGF-β1-RNAi細胞植入六至九週大且具正常免疫力的小黑鼠(C57BL/6)皮下，比較這些腫瘤細胞在小黑鼠皮下的生長情形。結果：與野生型腫瘤細胞(B16F0)或是載體控制組細胞(B16F0/vector-control)比較，B16F0/TGF-β1-RNAi細胞內轉型生長因子β1的表現明顯減少，這三株細胞在體外培養時生長速率差不多，在植入小鼠皮下後第十四天，植入野生型腫瘤細胞(B16F0)的小黑鼠腫瘤細胞其大小為495.32±77.25立方公厘，植入載體控制組細胞(B16F0/vector-control)的小黑鼠腫瘤細胞其大小為516.65±73.71立方公厘，植入B16F0/TGF-β1-RNAi細胞的小黑鼠腫瘤細胞其大小則只有326.72±97.34立方公厘，明顯降低許多，以one-way ANOVA統計分析P<0.05，在B16F0/TGF-β1-RNAi腫瘤內發現有明顯的CD4(上標 + )及CD8(上標 + )細胞浸潤，而且B16F0/TGF-β1RNAi腫瘤內微血管密度明顯降低。結論：我們所設計的轉型生長因子β1寡核?酸能抑制黑色素瘤細胞（B16F0細胞株）轉型生長因子β1的表現，利用核糖核酸干擾抑制技術來降低黑色素瘤轉型生長因子β1的產生，可以抑制此腫瘤細胞在小黑鼠皮下的生長，這個發現有助於未來腫瘤基因治療的應用。

This study examined the effects of college students' perception of PE teachers' leadership on exercise health belief, physical activity behavior and intention. There were 485 college students (male 237, female 248) used as the subjects. All the subjects were administered to the PE Teachers' Leadership Inventory, Exercise Health Belief Inventory, Physical Activity Behavior Questionnaire, and Physical Activity Intention Scale. The collected data were conducted by path analyses. The findings were as follows. First, the behavior of training and instruction and the behavior of administration could directly predict the exercise perceived benefits. The behavior of appreciation and the behavior of communication could directly predict cues to action. Second, the perceived exercise barriers and cues to action could directly predict the physical activity behavior. Third, the perceived exercise barriers and the perceived exercise benefits could directly predict physical activity intention, The results could be used as reference for PE teachers in teaching, promoting the students' exercise health beliefs, and improving their exercise intention and body physical activity behavior.