歐黴素及紅黴素為人畜共通用藥，屬於14環巨環黴素類抗生素 (Macrolides)，為中廣效性抗生素。本研究建立之歐黴素及紅黴素檢驗方法，移動相採用0.1%甲酸水溶液與乙腈 (70：30, v/v) 之混合液，以流速0.2mL/min、注射量10 μL，經Thermo BDS HYPERSIL C18分析管柱 (2.1 × 50 mm, 3.0 μm) 層析後進入串聯質譜儀進行分析，歐黴素及紅黴素經由電灑游離正離子離子化可得母離子分別為m/z 688 及734，歐黴素主要生成子離子片段為m/z 158、544，紅黴素為m/z 158、576，利用離子片段訊號之滯留時間、訊號面積及特徵子離子片段強度比，可做為歐黴素及紅黴素殘留檢測定性及定量之依據。萃取淨化之方法，經測試以0.2%偏磷酸
與甲醇 (6：4, v/v) 之混合液，比較三種固相萃取匣 (Oasis HLB, Oasis MAX及C18)，結果以利用Oasis HLB固相萃取匣萃取及分析，可得到最佳之回收率及淨化效果，且具有良好之再現性。本方法在歐黴素及紅黴素檢出限量均為0.01 μg/g (ppm)，平均回收率78.2%~86.4%。

Oleandomycin and erythromycin are 14-membered ring macrolide antibiotics. A simple and reliable method using liquid chromatography- tandem mass spectrometry had been developed for determination of oleandomycin and erythromycin in beef, pork, chicken and sheep. The LC separation was performed on Thermo BDS HYPERSIL C18 column (2.1 × 50 mm, 3.0 μm) with 0.1% formic acid/acetonitrile (70: 30, v/v) as the mobile phase at the flow rate 0.2 mL/min. The electrospray positive ionization produced
the molecular related ions at m/z 688 and 734 for oleandomycin and erythromycin, respectively. Two characteristic transition reactions (m/z 688→158, 544; m/z 734→158, 576) in selected reaction monitoring
were tested for detection and confirmation of oleandomycin and erythromycin, simultaneously. The analytes were extracted with 0.2% metaphosphoric acid/methanol (6 : 4, v/v) then cleaned up by three solid-phase extraction cartridges (Oasis HLB, Oasis MAX and C18), and had better results from Oasis HLB cartridges. The recoveries for oleandomycin and erythromycin analysis by developed method were 78.2%~86.4%. The
limits of determination were 0.01 μg/g (ppm) for both oleandomycin and erythromycin analysis.