利用包括水蒸汽蒸餾 (SD)，三種不同壓力下 (2000、3000及4000 psi) 超臨界二氧化碳 (SC-CO2)、正己烷、乙酸乙酯及甲醇萃取肉豆蔻粉末，上述七種萃取物利用硫氰酸鐵法檢測抗氧化性，對照抗氧化劑為BHA及α-生育醇，結果得知其抗氧化性依序為BHA > 甲醇萃取物 > 乙酸乙酯萃取物 > α-生育醇 > 正己烷萃取物> SC-CO2 3000 psi > SC-CO2 4000 psi > SC-CO2 2000 psi > SD。 SC-CO2萃取物及SD精油等進一步利用GC及GC/MS進行成分鑑定，結果顯示其內酚類化合物以SC-CO2 2000 psi萃取物最高，SD萃取物最低。單萜烯化合物含量以SD萃取物最高，SC-CO2 4000 psi最低。由上述結果可推測肉豆蔻揮發性成分萃取物中，水蒸汽蒸餾精油及超臨界二氧化碳萃取物的抗氧化性同時受到酚類化合物及單萜烯化合物的影響。

Seven extracts of nutmeg, including 1 essential oil using steam-distillation (SD), 3 extracts using supercritical carbon dioxide (SC-CO2) extraction at 3 different pressures (2000, 3000 and 4000 psi) and 3
solvent extracts using n-hexane, ethyl acetate and methanol, respectively, were obtained to evaluate their antioxidant properties. The antioxidant activities of these materials were determined and compared with antioxidants BHA and α-tocopherol in vitro using a ferric thiocyanate assay. The antioxidant properties of these extracts and antioxidants were determined as follows. BHA > methanol > ethyl acetate > α-tocopherol > n-hexane > SC-CO2 3000 psi > SD > SC-CO2 4000 psi > SC-CO2 2000 psi. In these extracts, the volatile compositions from the SC-CO2 and SD extracts were further identified by GC and GC/MS. The total
concentration of phenolic compounds was found to be the highest in SC-CO2, 2000 psi extracts, while the lowest total concentration was found in the SD extracts. The highest concentration of monoterpenes was found
in the SD extracts, while the lowest concentrations were found in the SC-CO2, 4000 psi extracts. These results suggest that the antioxidant activities of nutmeg extract by steam-distillation and SC-CO2 extraction were not only affected by the amounts of phenolic compounds but also the amounts of monoterpenes.