篇名 | 人腦出血血腫周圍神經元凋亡及部分調控基因的研究 |
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卷期 | 20:5 |
並列篇名 | Neural Apoptosis of Around Hematoma and Some Apoptosis-gene in Intracerebral Hemorrhage Patients |
作者 | 吳成翰 、 丁喜豔 、 葉小包 、 王海燕 、 黃繩躍 、 黃愛民 、 李會忠 、 吳松鷹 、 余吉 、 嚴曉華 |
頁次 | 440-446 |
關鍵字 | 人 、 Human 、 腦出血 、 Intracerebral hemorrhage 、 血腫 、 Hematoma 、 細胞凋亡 、 Cell apoptosis 、 凋亡基因 、 Apoptosis gene 、 Scopus 、 TSCI |
出刊日期 | 200910 |
觀察腦出血(ICH)遲發性神經元死亡現象,瞭解人腦出血後血腫周圍組織神經元凋亡及相關調節機制,為臨床治療腦出血繼發性損傷尋找新的方法。對29例高血壓腦出血血腫周圍腦組織病理標本和6例非正常死亡3小時內所取的腦組織標本,採用去氧核糖核酸末端轉移介導的缺口末端標記法(TUNEL)檢測神經細胞凋亡率;用免疫組織化學法檢測Bcl-2、Bax、P53、Caspase-3蛋白表達水準。腦出血患者血腫周圍組織細胞凋亡率及Bcl-2、Bax、P53、Caspase-3蛋白表達明顯高於正常對照組,差異有統計學意義(P <0.01)。Bcl-2 、P53蛋白表達與細胞凋亡率呈負相關;Bax、Caspase-3蛋白表達及Bax/Bcl-2與細胞凋亡率呈正相關。細胞凋亡機制參與了腦出血後繼發性神經元損傷。人腦出血後造成Bcl-2、Bax、P53蛋白表達增加,Caspase-3峻酶活性增加。
To observe human neural apoptosis and expression of apoptosis-related genes in order to elucidate regulation mechanism in the perihematomal region of Intracerebral Hemorrhage (ICH) patients. We selected 29
specimens of the perihematoma region from 29 patients who underwent surgical evacuation of an intracerebral hematoma.The control group included 6 samples that were collected from crops within 3 hours after they died from accidents. The neural apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine 5'triphosphate nick end labeling (TUNEL) method and the expression of Bcl-2, Bax, p53, and
caspase-3 genes were detected by immunohistochemistry method. The apoptosis rate and the expression of Bcl-2, Bax, p53, caspase-3 in the perihematomal region of ICH patients increased significantly in comparison
with the control group ( p <0.01). The expression levels of Bcl-2and p53 correlated negatively with the apoptosis rate ( p <0.01), while the expression levels of Bax , caspase-3 and the Bax/Bcl-2 ratio correlated positively with the apoptosis rate in perihematomal region of ICH patients ( p <0.01). Apoptosis was involved in the delayed brain
injure after ICH in human. The expression of Bax , caspase-3 and the Bax/Bcl-2 ratio changed as the apoptosis and may play an important role during the period of apoptosis.