細胞如何感知其外部環境的變化，並據以作出適當的對應，一直是細胞生理研究的熱門話題。人們試著操控物理性、化學性以及生物性等不同刺激方式，藉由細胞對這些刺激所產生的代謝、趨化、胞內分子調控以及凋亡等反應，企圖解開複雜的細胞生理反應機制。因應此一研究需求，各種不同細胞監測技術陸續被開發，用以觀察細胞內外不同的生理反應。本文回顧過往細胞檢測相關技術，就細胞分泌、蛋白質調控以及細胞力學等不同研究標的，比較各別檢測技術優勢以及使用限制，並探討不同方法相互整合可能產生的優點，嘗試歸納未來細胞監測技術整合與應用的趨勢。

Cells can sense chemical, biological and physical changes around their environment and regulate a proper response to different stimulus. It is an attractive topic for most researchers to elucidate different cell mechanisms based on the observation of cellular behaviors. People tried to induce various stimulations by using chemical reagents,
immunological proteins or mechanical indentation, and then cellular behaviors e.g. intracellular molecules modulation, cell movement, secretion were associated with these stimulus. Based on the established correlations,cell physiology was investigated and several hypotheses were proved. In the past year, several monitoring techniques were developed to meet different requirements of cell measurement. In this article, we reviewed the reported techniques and compared their application limitations, respectively. The advantages of integration with different technologies for cellular behaviors were discussed. Also, the future trend of cellular monitoring was proposed based on the reviewed evidences.