篇名 | Aflatoxin in Raw Peanut Kernels Marketed in Malaysia |
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卷期 | 18:1 |
作者 | SAHAR ARZANDEH 、 JINAP SELAMAT 、 HANIFAH LIOE |
頁次 | 44-50 |
關鍵字 | Aflatoxin 、 food safety 、 immunoaffinity clean-up 、 HPLC with fluorescence detection 、 peanut 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 201002 |
The occurrence of aflatoxin in eighty-four samples of raw peanut kernels which are randomly collected from Malaysian supermarkets was examined. Analysis for aflatoxin was performed by solvent extraction and immunoaffinity clean-up followed by the determination using high performance liquid chromatography equipped with post-column photochemical reactor for enhanced detection and fluorescence detector. A detection limit of 0.01-0.09 ng/mL and a quantification limit of 0.04-0.30 ng/mL were obtained. The aflatoxin concentrations ranged from not detected to 97.28 ng/g in all samples investigated. About 78.57% of the samples were contaminated with aflatoxin, of which 10.71% exceeded the maximum tolerable limit of 15 ng/g set by the Codex.
Average recoveries of the aflatoxin analysis were acceptable which were in the range of 74.85 ± 8.83% for AFG2 at the concentration
of 0.15 ng/mL and 103.91 ± 6.45% for AFB2 at the concentration of 0.15 ng/mL. The average daily intake estimated for total aflatoxins
was 10.69 ng/kg body weight. There was a significant difference (P < 0.05) in aflatoxin content between brands and locations.