篇名 | Effects of (-)-Epigallocatechin-3-gallate on Cyclooxygenase 2 and Matrix Metalloproteinase 3, 9, 13 Expression Induced by IL-1ß in Rat Meniscal Fibrochondrocytes |
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卷期 | 17:6 |
作者 | Lee, Herng-sheng 、 Huang, Hsiu-hui 、 Tsao, Tang-yi |
頁次 | 459-466 |
關鍵字 | epigallocatechin-3-gallate 、 IL-1b 、 fibrochondrocyte 、 meniscus 、 matrix metalloproteinase 、 cyclooxygenase 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200912 |
The objective of this study was to examine the effect of (-)-epigallocatechin-3-gallate (EGCG) on cyclooxygenase 2 (COX-2) and matrix metalloproteinase (MMP) 3, 9, and 13 expressions induced by IL-1b in rat meniscal fibrochondrocytes. Rat normal meniscal fibrochondrocytes were enzymatically isolated from meniscal tissue from the hind leg knee joint. Cells were pretreated with EGCG at a concentration of 50 mM to examine its effect in the presence of IL-1b insult. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR were used to assess the COX-2 and MMP3, 9, and 13 gene expressions, respectively.
COX-2 gene upregulation induced by IL-1b in primary cultures of rat meniscal fibrochondrocytes was significantly suppressed by EGCG (p < 0.05). MMP 3, 9, and 13 gene expressions were also significantly induced by IL-1b stimulation and significantly inhibited by EGCG treatment (p < 0.05). These results indicated that EGCG may be of value in the treatment of meniscal inflammation associated with IL-1b secretion.