本研究利用3T3-L1前脂肪細胞探討分離大豆蛋白(ISP) 水解物的促進脂解作用，以胞外的甘油釋放量(GR) 和胞內的三酸甘油酯殘留量(TR) 作為脂解程度的指標，較高的GR或較低的TR代表較佳的促進脂解活性。ISP經中性蛋白酶(Neutrase) 於45℃和pH 6.0條件下水解4h所得水解物(NH4h)，添加400-800 ppm NH4h與對照組比較均可顯著提升細胞培養液中的GR(p < 0.05)，同時其TR也有降低的趨勢。其中400 ppm濃度時就已具有最高的甘油釋放量，GR從79.03提升至109.24 nmol/ml，且其TR從3.06降至2.85 μmol/mg protein，兩者均確認NH4h具有促進脂肪細胞脂解作用的效果。其次，NH4h依序以30, 10和1 kDa分子量限值 (MWCO) 濾膜進行區分，其中以10 kDa MWCO濾膜濃縮而得的10 kDa concentrate，添加400 ppm於細胞培養液中與添加NH4h比較，結果其GR從108.86增加至114.88 nmol/ml，同時也能將TR從2.85降至2.35 μmol/mg protein。

The aim of this study was to explore the lipolysis-stimulating activity of isolated soy protein (ISP) hydrolysate using 3T3-L1 adipocytes. Extracellular glycerol release (GR) and intracellular triglyceride residual (TR) were employed as markers for lipolysis in cells. The higher GR or the lower TR represents better lipolysis-stimulating activity. ISP was hydrolyzed with Neutrase for 4 h to obtain the hydrolysate NH4h, which showed lipolysis-stimulating activity in adipocytes at 400-800 ppm levels. The highest GR was observed at 400 ppm. GR increased from 79.03 to 109.24 nmol/mL, and TR decreased from 3.06 to 2.85 μmol/mg protein when compared to control. Both markers showed the effcacy of NH4h to stimulate lipolysis in the cells. The 10 kDa concentrate obtained from the sequential fractionation of NH4h with 30, 10 and 1 kDa molecular weight cut-off membranes (MWCO) resulted in further enhancement of its lipolysis-stimulating activity in the cells. The GR increased from 108.86 to 114.88 nmol/mL, and TR decreased from 2.85 to 2.35 μmol/mg protein at the 400 ppm level (p < 0.05) when compared to the NH4h-added sample.