本文簡介一個利用微機電製程，依據免疫學之原理，利用微型磁珠結合專一性極高之抗體，以萃取特定病毒，並將其濃縮、純化、分離後，全自動進行聚合.連鎖反應之生物晶片系統平台。此微流體整合型系統藉由微幫浦與微閥門的作用下，能自動化地完成疾病診斷過程中之檢測流程，並可將整體之反應之時間由傳統檢測之約莫 8－10 小時縮短至 1.5 小時左右即可完成。本晶片系統已於臨床檢體中成功地純化及檢測出登革熱血清第一型至第四型病毒 (dengue virus serotype 1-4)，且其診斷之靈敏度約為 100 PFU/mL。本晶片系統所提供之快速自動化檢測平台，於基因分析、分子生物學以及快速感染性疾病之偵測將有極大之助益。

The current study reports an innovative miniature microfluidic system for rapid detection of infectious diseases.
Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses, the target virus can be recognized and further purified by a magnetic field generated by the built-in microcoils. The purified target virus can be then lysed to release ribonucleic acid (RNA) for the subsequent
nucleic acid amplification process such as reverse transcription polymerase chain reaction (RT-PCR) processes.
Experimental results showed that the dengue viruses (serotype 1-4) can be successfully purified and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of viral RNA can also be completed utilizing the on-chip temperature control module in an automatic manner with the
sensitivity as high as 100 PFU/mL. As a whole, the developed system may provide a potential micro-total-analysissystem (m -TAS) for bio-sample pre-treatment and the fast diagnosis of infectious diseases.