成像質譜術結合微區取樣 (micro-area sampling)、化學分析 (chemical analysis) 與空間解析的能力，可提供樣品原子 (或分子) 組成與空間分布關聯性的資訊，逐漸成為生醫研究領域中不可或缺的重要分析工具。
本文依序介紹各原子 (或分子) 成像質譜術 (二次離子質譜術、基質輔助雷射脫附游離質譜術、雷射剝蝕感應耦合電漿質譜術) 之基本原理，簡述其於生醫領域之研究現況及國內現有相關硬體資源，以供感興趣讀者對此一新興研究領域之參考。?

Imaging mass spectrometry combining the ability of micro-area sampling, chemical analysis, and spatial resolution is capable of providing spatially-resolved atomic (or molecular) composition information, which makes it becoming an indispensible research tool for biomedical fields. Herein, we briefly introduce the principles of imaging mass
spectrometry including secondary ionization mass spectrometry (SIMS), matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS)
first, followed by reviewing its current applications in biomedical researches.