癌症的治療，放射治療一直扮演著重要的角色，隨著科學的快速發展，放射治療的技術也有長足的進步。唯有採用適當的輻射劑量，方能有效治療並避免腫瘤患者的不適。為了建立醫療劑量的使用模式，必須準確偵測醫用輻射劑量，傳統上使用游離腔、底片、半導體二極體、熱發光劑量計等物理性變化設備，做為劑量驗證的工具。本研究提出新型醫用之有機性輻射劑量計，藉由輻射劑量之生化偵測模式，提供臨床使用的另一項選擇。本研究中採用木質纖維素、有機染料與蛋白質酵素作為標的，經由輻射照射後改變結構或產生游離之化合物，藉由高效能液相層析偵測儀與酵素分析法進行分析，取得解離化合物濃度、種類與輻射累積劑量之關係，建立輻射劑量之
生化偵測模式。此生物標的之變化量可作為醫用輻射劑量的定量依據，並建立兩者相關性，提供醫療照射劑量的參考。

Cancer is the major cause of death in recent years in Taiwan. For cancer patients, radiation therapy is one of the most important therapeutic modalities. Modern radiation therapy continues to progress at an unprecedented rate. This development is strongly related to the evolution of the corresponding advances in the computer and physical technology. The goals of radiation therapy have always been to maximize the
probability of controlling the tumor and minimizing normal tissue complications. To achieve the goals of disease curing and pain relief of cancer patients, radiotherapy with appropriate radiation doses is basically required. It is necessary for precise dosimetry to clarify the reference between doses usage and curing performance. Farmer type ionization chamber, film, silicon diode detector, and thermoluminescence dosimeter（TLD）are usually used as the tools of dosimetry traditionally. An innovative clinical bio-dosimeter to detect the bio-response of radiation is proposed in this study. Lignocellulose, specified enzyme and synthesized dye were examined as the radiation-related target. Result shows that the lignocellulose unchains the certain molecular bonds and release free ionizing glucose or xylose after the target being radiated. Concentrations of these free sugars were analyzed by HPLC (high performance liquid chromatography). Mechanism of lignocellulose decomposition will be studied in the future. Enzymatic catalysis of radiated glucose oxidase is slightly different from that of normal one. Synthesized dye (X) is good target for the indication of dosimetry. A linear relationship between of radiation dose and the optical density of radiated X at 642 nm is built up. The linear regression will be reliable for the clinical application. The bio-response would refer to clinical use and quantitative determination of the doses of radiation.