研究目的：台灣醫學中心員工除了大量的工作外還需負擔臨床教學及研究活動等業務，因此普遍工作壓力偏高。先前所作的工作壓力分析研究主要針對醫療人員和護理人員，往往忽略其他部門的員工。因此，本研究針對醫學中心員工進行工作壓力分析，以探討醫學中心各部門員工壓力的差異。研究方法：自2008年7月1日至2008年8月31日於南部某醫學中心以隨機取樣的方式，經個案同意後進行問卷填寫。所有受試者填寫一般問卷以獲得基本人口學資料，並填寫抽菸、喝酒、運動狀況相關資料及工作內涵問卷(Job Content Questionnaire, JCQ)。本研究共收集1770份有效問卷，以描述性統計分析人口學特性；以獨立變項t檢定及卡方檢定(Chi-square)，比較高工作壓力組與低工作壓力組之間的差異；以單變項邏輯迴歸模式(univariate logistic regression)及多變項邏輯迴歸模式(multiple logistic regression)分析工作壓力的危險因子。研究結果：本研究結果顯示工作壓力和年齡、性別、職務分類、職務上照顧病患及工作輪班方式之間有相關(P<0.01)。以多變項邏輯迴歸分析發現，工作年資1-5年(AOR=1.79, 95%CI=1.13-2.83)、工作年資6-10年(AOR-2.15, 95%CI=1.25-3.69)、工作年資＞10年(AOR=2.96, 95%CI=1.65-5.29)、學歷研究所含以上(AOR=2.27, 95%CI=1.20-4.31)、輪班工作者(AOR=1.65, 95%CI=1.19-2.30)、醫療人員(AOR=1.58, 95%CI=1.01-2.48)和護理人員(AOR=2.12, 95%CI=1.50-2.99)為獨立的危險因子。年齡40-44歲(AOR=0.45, 95%CI=0.23-0.88)、≧45歲(AOR=0.40, 95%CI=0.19-0.82)及管理階層者(AOR=0.36, 95%CI=0.14-0.95)為工作壓力保護因子。結論：工作壓力與輪班、正常班偶輪班、工作年資、高學歷（研究所含以上）、護理人員及醫療人員有相關。本結果可提供不同的工作部門，作為未來改善員工工作壓力進而提高工作績效之參考。

Objective: The work stress among the staff at medical centers in Taiwan is high because of the large service volume, teaching loads, and research activities. Previous studies on this issue have been focused on doctors and nurses and left out other staff members. Therefore, we conducted a study to evaluate the overall work stress at a medical center. Materials and Methods: We recruited workers working in a medical center in southern Taiwan between July 1 and August 31, 2008. Information on demographic characteristics was collected through a self-administrated questionnaire. Participants also reported habits of smoking, drinking, and regular exercise and completed a job content questionnaire. Results: There were 1770 workers participated in this study. We observed positive associations between work stress and age, gender, work category, in charge of patient care, shift work (all with p<0.01). After adjusting for other factors, we found a work history of 1-5 years (adjusted odds ratio [AOR]=1.79, 95% confidence interval [CI]: 1.13-2.83), a work history of 6-10 years (AOR=2.15, 95%CI=1.25-3.69), and a work history of >10 years (AOR=2.96, 95%CI=1.65-5.29), high education (AOR=2.27, 95%CI=1.20-4.31), shift work (AOR=1.65, 95%CI=1.19-2.30) and working as a nurse (AOR=2.12, 95%CI=1.50-2.99) and a physican (AOR=1.58, 95%CI=1.01-2.48) were independent risk factors of work stress. While age of 40-44 years (AOR=0.45, 95%CI=0.23-0.88), age>45 years (AOR=0.40, 95%CI=0.19-0.82) and administration (AOR=0.36, 95%CI=0.14-0.95) were protective factors. Conclusion: At the medical center, work stress is associated with seniority, age, shift work, and job category. Therefore, different strategies should be applied to intervention for work stress control.