本試驗利用多種抗氧化分析方法與Ames test，測試並評估靈芝乙醇萃取物(Ganoderma lucidum (Leyss ex. Fr.) Karst ethanolic extract, GLEE)之體外抗氧化與抗致突變性。結果顯示：一、抗氧化方面：以ferric thiocyanate method與conjugated diene method測試，於20 mg/mL濃度下，GLEE展現最高的總抗氧化能力(91.4%)與抗氧化活性(74.2%)。在以不同之催化因子所催化之脂質過氧化系統中，GLEE均會表現出不同程度之抗氧化性，並以在自氧化系統中有最佳之抗氧化性，且在各系統中，脂質過氧化抑制效果會隨著GLEE濃度之增加而增加。二、毒性試驗方面：對TA98菌株，GLEE於未加S9 mix時，其5 mg/plate菌落數與對照組有顯著差異，表示其對菌株具毒性，但減至2.5 mg/plate則不具毒性；對TA100與TA1535二菌株則皆不具毒性。三、致突變性試驗方面：各GLEE測試劑量對參試菌株均不具致突變性。四、抗致突變性試驗方面：當GLEE濃度為5 mg/plate時，當有NQNO存在時，對TA98菌株有最佳的抗致突變性，抑制率可達到92%，而對TA1535菌株的抗致突變性最差，抑制率可達到81%；當有B[a]P存在時，對TA1535菌株有最佳的抗致突變性，抑制率可達到76%，而對TA100菌株的抗致突變性最差，抑制率僅可達到21%。

The study was investigated the antioxidant and antimutagenic activity of
Ganoderma lucidum (Leyss ex. Fr.) Karst ethanolic extract (GLEE) by several antioxidant activity determinated methods and Ames test. Results indicate that: 1. Antioxidant activity: Results indicated that the GLEE showed a high total antioxidant capacity (91.4%) with ferric thiocyanate method and exhibited the highest antioxidant activity (74.2%) with conjugated diene method at 20 mg/mL. The GLEE exhibited various degree of antioxidative activities and the linoleic acid peroxidation inhibition
percentage of GLEE was increased with increasing concentrations at all different proxidants systems. The height inhibition was showed in the autoxidation system. 2. Toxic test: Non-toxicity was found in GLEE with or without S9 mix toward TA100 and TA1535 at the dosage of 5 mg/plate. Non-toxicity was also found in GLEE without S9 mix toward TA98 at 2.5 mg/plate. 2. Mutagenic test: Non-mutagenicity was found in GLEE with or without toward TA98, TA100 and TA1535. 3. Antimutagenic test: GLEE (at 5 mg/plate) showed the strongest inhibition effect toward TA98 against NQNO (the inhibition was 92%) and toward TA1535 (the inhibition was 76%) against B[a]P, but has lower exhibition effect toward TA1535 (the inhibition was 81%) against NQNO and toward TA100 (the inhibition was 21%)
against B [a] P, respectively.