A simple and fast micellar liquid chromatographic (MLC) method for simultaneous analysis of the antibiotic fungicides of blasticidinS and kasugamycin was developed. Chromatographic separation was achieved on a Phenomenex® Aqua 5μ C18 125 A column. Themobile phase consisted of an aqueous mixture of 69.3 mM sodium dodecyl sulfate (SDS) with deionized water (50 : 1, v/w). The flowrate was set at 1.0 mL/min, and the absorbance was monitored at 210 nm with a photodiode array detector. The total run time was lessthan10 min, and the retention times (tr) were 2.47 min for blasticidin S ion, and 7.15 min for kasugamycin ion. Good linear relationshipswere obtained with correlation of coefficients (r) of 0.997 for kasugamycin, and 0.999 for blasticidin S. The limits of detection (LODs)of blasticidin S and kasugamycin were 0.5 μg/mL and 0.75 μg/mL, respectively, and the limits of quantitation (LOQs) for blasticidinS and kasugamycin were 1.5 μg/mL and 2.2 μg/mL, respectively. The recoveries of blasticidin S and kasugamycin in blank irrigationwater ranged from 94.9 - 97.3% and 87.8 - 99.7%, respectively. By using MLC method blasticidin S and kasugamycin could be analyzedsimultaneously.