人工牙根表面與骨組織形成良好的骨整合是植牙成功的關鍵因素之ㄧ，而除了形態設計與手術技巧的影響外，人工牙根骨整合的速率及質量與表面性質息息相關。透過不同的表面處理方式改變人工牙根的表面性質，可能有助於加速骨整合。影響植體-組織界面表面性質的因子保括表面組成份、表面形貌、粗糙度、親水性及滅菌方法等，有許多學者針對以上不同表面性質對骨整合的影響做過體外研究。然而，大多束文獻皆以處理過的平板試片模擬人工牙根表面作為試驗模型，因此對人工牙根本身型態設計對後續表面處理及細胞本身行為的影響，並無法完整地模擬之。本研究的目的就是使用一種常見的類骨母細胞-人類骨腫瘤細胞株MG63作為細胞模型，觀察MG63細胞再金屬中心自行研發的商用四級純鈦製人工牙根之體外細胞親和性。以機械加工表面人工牙根作為控制組，針對鹼蝕熱生物活性化表面處理人工牙根進行類骨母細胞在人工牙根表面貼上附及增生表現之比較，以評估其體外細胞親和性良窳。實驗結果顯示:細胞初期貼附數量在人工牙根的各幾何部位有所不同，粗螺紋區>切削刃區>根端區>細螺紋區。生物活性化表面處理人工牙根比機械加工表面人工牙根具有更良好的細胞貼附型態與更加的細胞增生活性，尤其在細螺紋區的細胞貼附數量及形貌，兩個組別有很大的差異。故由類骨母細胞早期貼附與增生情形看來，生物活性化表面處理製成確在人工牙根上達到良好的表面改質效果。

One of the key factors for the success of dental implants is the achievement of close contact between the bone and the implant surface. This is what is known as osseointegration. Besides implant design and surgical technique, the rate and quality of osseointegration in dental implants are related to their surface properties. Osseointegration may be accelerated by modifying tehe surface properties with different surface treatments. Surface composition, roughness, hydrophilicity and sterilization methods are parameters that influence surface properties of implant-tissue interation. There were many in-vitro studies reviewed about the influence of different implant surface properties on osseointegration. However, most of them used treated-plate models to simulate implant surfaces. Treated-plate models cannot simulate the influence of implant design upon followed surface treatment and cellular behavior. The aim of the present study was to investigate the cytocompatibility in vitro directly upon CP4 Titanium dental implants made by MIRDC using MG63 human osteosarcoma cells as a cellular model. The in vitro cytocompatibility characterized by cell morphology, adhesion ,and proliferation activity were assessed by comparing the bioactive alkali-acid and heat(AAH) treatment to machined surface as control. The in vitro study showed the numbers of early attached cells on different geometric regions of implants were different:macro-screw region > cutting edge region > apex region > micro-screw region. Enhanced osteoblast adhesion and higher proliferation activity for the bioactively modified implants compared to the machined-surface implants, especially on the micro-screw region. In conclusion, the AAH surface treatment on dental implants indeed enhanced the biological response in vitro, promoting an early osteoblast-like cell adhesion and proliferation.