本研究去探討小柴胡對膽汁鬱積所造成脂質過氧化，是否有抗氧化的作用。對於肝纖維化的起始及重要調控中心－貯脂細胞(Ito cell)，則以西方墨點法(western blotting)去探討小柴胡湯在貯脂細胞的蛋白質階層，是否有抑制作用。結果顯示：小柴胡湯對膽道結紮六週後的脂質過氧化及貯脂細胞在蛋白質階層的表現皆有抑制作用。因此推論小柴胡燙手預防及對抗肝纖維化的作用，與其抗氧化及其抑制貯脂細胞在蛋白質階層的表現有關。

The Ito cell has the central role in the process of fibrogenesis. Bile duct ligation induced cholestasis that caused oxidant stress and stimulated fibrogenesis in the liver. Thus, the objective of this study was to determine whether the Xiao-Chai-Hu-Tang exerted its anti-oxidant effect and suppressing effect on Ito cell. Results suggested that Xiao-Chai-Hu-Tang prevented liver fibrosis through the mechanism of anti-oxidation and suppression of Ito cell.