目的：基因體結構變異為腫瘤形成的表徵之一。於本實驗室先前的研究，我們針對肺癌族群進行微矩陣比較基因體的雜交分析 (array-CGH)，其結果顯示 PAFAH1B1 基因具有基因體結構擴增的現象。因此，我們推測 PAFAH1B1 基因為肺癌有潛力的致癌基因。材料與方法：本研究利用即時定量反轉錄 PCR (q-RT PCR) 及免疫組織染色法 (IHC) 對91 位肺癌族群檢測 PAFAH1B1 基因其 mRNA 及蛋白層次變異情形。結果：PAFAH1B1 基因於 mRNA 層次的過度表現頻率為61.5%，於蛋白層次過度表現頻率為56%，且此基因於mRNA 及蛋白層次的過度表現皆與病人的晚期具有相關性 (mRNA：P=0.001，蛋白層：P=0.05)，且屬於腺細胞癌的病人於蛋白層次的過度表現具有較差的預後 (P=0.049)。結論：其結果顯示PAFAH1B1 基因於肺癌族群具有過度表達的變異，且可能參與肺癌細胞轉移過程。

Rationale and Objectives: Genomic DNA copy number variation is a hallmark of cancer. In our previous array-comparative genomic hybridization (array-CGH) study, we showed that PAFAH1B1 was amplified in lung cancer patients, suggesting that PAFAH1B1 is a potential oncogene in lung cancer.
Methods: In this study, we have determined the mRNA and protein expression level of PAFAH1B1 in 91 lung cancer patients using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and
immunohistochemistry (IHC). Main Results: The PAFAH1B1 mRNA and protein overexpression frequency were 61.5% (56/91) and 56% (51/91) in lung cancer patients. The results indicated that mRNA and protein overexpression level of PAFAH1B1 was significantly associated with late stage (mRNA:P=0.001, protein: P=0.05) and poor survival in lung adenocarcinoma (P=0.049). Conclusions: The results revealed the roles of overexpressed PAFAH1B1 in tumor progression and poor survival in lung cancer.