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台北海洋技術學院學報
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| 篇名 | 光度、溫度及鹽度對螺旋藻超氧歧化酵素產量及抗氧化之影響 |
|---|---|
| 卷期 | 5:1 |
| 並列篇名 | The Effects of Light Intensity, Temperature and Salinity on the Production of Superoxide Dismutase and Antioxidation of Spirulina Platensis |
| 作者 | 聶方珮 、 孫明輝 、 林英才 、 林雪良 、 鄭明得 、 彭清勇 |
| 頁次 | 044-060 |
| 關鍵字 | 螺旋藻 、 抗氧化能力 、 超氧歧化酵素 、 NBT光還原反應法 、 SOD 、 Spirulina platensis 、 Antioxidative activity 、 Superoxide dismutase 、 NBT test |
| 出刊日期 | 201203 |
中文摘要
螺旋藻(Spirulina platensis)被發現含有高量抗氧化物、維生素A 和β-胡蘿蔔素及超氧歧化酵素(superoxide dismutase, SOD)等，可幫助人體降低自由基的傷害，因此成為保健機能食品市場的熱門標的。本研究擬探討不同光照度、溫度及鹽度等培養條件對螺旋藻清除自由基DPPH 及超氧陰離子活性的影響。實驗結果顯示最適螺旋藻培養條件為光照強度介於2000~4000 Lux、溫度介於30~37℃及鹽度0.1M 以下之環境，培養42 天後的螺旋藻最高生質產量可達6.0 g/L；此外，藻體甲醇萃取物清除自由基DPPH 及超氧陰離子的能力測試結果顯示自由基清除率最高分別可達51.3%及34.5%，具有開發為高經濟與高營養價值保健品之潛力。
英文摘要
Spirulina platensi has been the candidate for commercial high-quality health food, due to it has high content of antioxidant, vitamin A, β-carotenoid and superoxide dismutase (SOD). This study was focus at the
effects of varied culture conditions including light intensity, temperature,and salinities on the growth and the antioxidative activity of Spirulina platensi.The results indicated that the optimum culture conditions for the growth of Spirulina platensi were as follows, 2000-4000 Lux of light intensity, 30-37℃ and lower than 0.1M of salinity. After 42 days cultivation, the max bio-mass will be more than 6.0 g/L in dry based. Additionally, the scavenging activity of the methanol extract of Spirulina platensi for DPPH and superoxide free radicals will be 51.3% and 34.5%, respectively. From this study, the Spirulina platensi could be
provided as an effective antioxidant and further developed as health food.