篇名 | Investigation of Neurochemical Techniques for Assessment of Nerve Regeneration within Polymer Guides |
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卷期 | 32:2 |
作者 | Chung-Chia Chen 、 Chu-Ying Chan 、 Li-Shu Chen 、 Fuu-Jen Tsai 、 Chun-Hsu Yao 、 Chin-Chuan Tsai 、 Yueh-Sheng Chen |
頁次 | 131-138 |
關鍵字 | Nerve injury 、 Nerve guide 、 Nerve regeneration 、 Tissue engineering 、 EI 、 SCI |
出刊日期 | 201204 |
The use of proteins associated with neurite outgrowth, synapse formation, and Schwann cell proliferation as surrogate measures for peripheral nerve regeneration is evaluated. Rat sciatic nerve regeneration across a 10-mm gap in silicone rubber conduits (SRCs), genipin-crosslinked gelatin conduits (GGCs), and porous GGCs (PGGCs) is observed. The axonal growth of regenerated nerves after 8 weeks is determined using light microscopy and computer-based quantitative image analysis. The expressions of axonal growth-associated protein 43 (GAP-43), synaptic protein synapsin I, and transforming growth factor type-β (TGF-β) in regenerated nerves are assessed using Western blot analysis. The nerve regenerates in the SRCs has a larger area and more myelinated axons compared to those in the PGGCs and the GGCs. The levels of GA P-43 and synapsin I, but not TGF-[l. are well correlated with axonal growth in the regenerated nerves. These data suggest that SRCs provide a more stable and suitable growth environment for regenerating axons than do PGGCs and GGCs. In addition, GAP-43 and synapsin I may be useful indicators of the status of regenerating axons in bridging conduits.