本文首次建立了一種簡單、快速、有效的分析方法，同時測定膽木葉中的5個特征化學成分的含量，其中包括3個生物鹼（短小蛇根草苷、10-羥基異長春花苷內酰胺和異長春花苷內酰胺）和2個黃酮（蘆丁和山奈酚-3-O- 蕓香糖苷）。色譜條件為Lichrospher 的C18色譜柱，以（A）0.1%磷酸水溶液和（B）乙腈為流動相，流速為1.0 mL / min，柱溫為30°C，檢測波長為226 nm。該方法系統適用性，線性關係，精密度，穩定性均良好，平均回收率為98.0 - 100.44%（r > 0.9993）。本文同時用該方法成功測定了10批不同採收期膽木葉的5個化合物含量，實驗結果表明該方法簡單可靠，能有效地用於膽木葉的質量評價。

A simple, rapid and validated method was developed for the simultaneous determination of five characteristic components, including three alkaloids (pumiloside, 10–hydroxy strictosamide, and strictosamide), and two flavonoids (rutin and kaempferol-3-O-rutinoside), in the Nauclea officinalis leaves. The chromatographic separation was achieved with a lichrospher C-18 analytical column by gradient elution with (A) 0.1% aqueous phosphoric acid and (B) acetonitrile as mobile phase, with a flow rate of 1.0 mL/min. The column temperature was set at 30°C. Detection was carried out on a photodiode array detector at 226 nm. The method was validated for system suitability, precision, accuracy and linearity. The average recovery of the method was 98.00 - 100.44% and linearity (r) was>0.9993. Furthermore, the method was successfully applied to the simultaneous determination of five analytes in 10 samples of N. officinalis leaves from different harvesting time of the herb. The results suggested that the method could be efficiently employed to evaluate the quality of N. officinalis leaves from various sources, which would benefit its quality control.