本研究目的為探討香菇中之多醣的萃取條件及理化特性分析，以作為開發菇類多醣產品的基礎應用。研究材料香菇為271品系，經高速均質處理前後在80、90及100℃熱水萃取一、二與三小時，發現其多醣萃取率高於未高速均質組，最高可增加3.4倍。將上述萃取所得的粗多醣，分析其多糖含量、黏度及分子量分布等，結果顯示香菇粗多醣的多醣含量為28.61-128.69mg/g（dry basis）。香菇粗多醣的黏度（測定濃度為10mg/mL）及分子量範圍，分別為0-16.7 Cp（centi poise）及120-540kDa。

The purpose of this study was to investigate the extraction conditions and the physicochemical properties of the crude polysaccharides in shiitake (Lentinula edodes), as the application of the polysaccharide products. The studying material was strain 271 of the L. edodes. After treating with high speed homogenizer, the L. edodes were extracted at 80, 90 and 100℃ for 1 to 3h. It was found that the highest yield of polysaccharides could raise 3.4 times than that of untreated samples. The crude polysaccharides were then tested for the polysaccharide content, viscosity and molecular weight distribution. The polysaccharides contents of the crude polysaccharides in L. edodes were 28.61-128.69mg/g sample (dry basis). The viscosity (measured concentration of 10mg/mL) and molecular weight of the crude polysaccharides were 0-16.7cP and 120-540 kDa, respectively.