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| 篇名 | 以RAPD及NBS專一性引子擴增之DNA標誌探討芒果品種間之遺傳相似度 |
|---|---|
| 卷期 | 58:4 |
| 並列篇名 | Genetic Similarity Analysis among Mangifera indica Cultivars / Lines Based on DNA Markers Amplified by RAPD and Specific NBS Primer |
| 作者 | 黃士穎 、 張錦興 、 林冠宏 |
| 頁次 | 285-294 |
| 關鍵字 | 漆樹科 、 核苷酸結合富含白胺酸重複序列 、 分子標誌 、 親緣關係圖 、 品種鑑定 、 Anacardiaceae 、 nucleotide binding leucine-rich repeat 、 DNA marker 、 phylogenetic tree 、 variety identification |
| 出刊日期 | 201212 |
中文摘要
本研究以抗病基因中最主要之核苷酸結合富含白胺酸重複序列(nucleotide
binding leucine-rich repeat, NBS-LRR)類型為分析對象 ，利用 15對randomly amplified
polymorphic DNA (RAPD)引子與NBS專一引子組合，對 32個芒果品種/系之DNA
進行polymerase chain reaction (PCR)，擴增出含有NBS區域之DNA序列，得到83
個抗病基因區域之多型性條帶。經 unweighted pair-group method using arithmetic
averages (UPGMA)及neighbor joining (NJ)分析，32個品種/系NBS類抗病基因型之
遺傳相似度介於 0.64-0.84 間，可分為 5 大群，作為芒果 NBS 類抗病基因型分類的
依據。本試驗結果所得之專一性分子標誌可提供芒果栽培品種鑑別之工具。
英文摘要
The objective of this study was to analyze the nucleotide binding leucine-rich repeat (NBS-LRR) in
analog resistance gene family in Mangifera indica using randomly amplified polymorphic DNA (RAPD)
markers. The combinations of 15 RAPD primers and 1 NBS specific primer were used to amplify NBS
sequences complementary to the DNA sequences from 32 cultivars / lines. Eighty-three polymorphic bands
of the NBS-LRR resistance gene analogs were obtained. The data were then analyzed by unweighted
pair-group method using arithmetic averages (UPGMA) and neighbor joining (NJ) programs. Five groups
were clustered ranging from 0.64 to 0.84 of similarity coefficient, indicating that NBS might be used for
phylogeny of the analog resistant gene in mango. Some specific polymorphic bands obtained in this study
could be used for mango cultivar identifications.