本硏究利用農業廢棄物油菜籽粕作爲基質，由本實驗室所購得的3株菌種所篩出的Bacillus subtilis (A)作爲水解菌種，以所馴養的醱酵產氫菌做爲產氫菌，進行一連串的水解串連醱酵 產氫的兩段式反應槽連續試驗，以評估油菜籽粕之厭氧產氫之可行性。
本硏究的試驗結果顯示，在相同總循序時間+水力停留時間下（12+24hr)，以分離二段式 反應槽SBR串聯CSTR，有最佳的單位體積反應槽每天產氫率（23.7 mmole-H2 /L • day)，優 於ASBR串聯CSTR，再優於共培養ASBR反應槽。不同起始pH分離培養反應槽SBR串聯 CSTR，前段SBR的起始pH値爲7.0時（出流水pH 6.82 )，有最佳的水解效果，溶解性COD 增加率爲17.8%;後段的CSTR醱酵產氫反應槽，當進流水pH =5.81 (出流水接近於5.0-5.5)
時，有最佳的單位體積反應槽每天產氫率爲12.1 mmole-H2 /L • day。
本硏究以水解SBR反應槽串聯醱酵產氫CSTR反應槽，其產氫量比單段反應槽之醱酵產 氫率較佳’可得知油菜籽粕基質經由SBR水解後’可以有效提升產氫率’且油菜籽粕取得容易，所以利用油菜籽粕基質來產氫的可行性高。

Rape-seed dregs, which are generating significant agricultural waste yearly, were used as a substrate in our study. Bacillus subtilis (A) screened as out hydrolytic bacteria bought from BCRC, together with the fermentative hydrogenic bacteria cultivated in our laboratory as hydrogenic bacteria, was used in a series and in continuous input tests of two-stages reactors for hydrolysis and fermentative hydrogenesis to evaluate the feasibility of fermentative hydrogenesis of rape-seed dregs.
The results show that with the same progressive times ( 12+24h) , the optimal daily hydrogen produced by ASBR+CSTR, and greater than the co-culturing ASBR reactor. For the separated reactor SBR+CSTR with different initial pH, the most optimal hydrolysis was obtained and the increase rate of soluble COD was approximately 17.8% when the initial pH of the preceding SBR was 7.0 (effluent with pH 6.82 ). For the posterior fermentative hydrogen producing CSTR, the optimal daily hydrogen attained 12.1 mmole-H2 /L when the influent pH was 5.81 (influent pH approximately 5.0-5.5).
The hydrogen production of the two-stage reactors was significantly higher than that of the single stage reactor. The rape-seed dregs substrate hydrolysis can be effectively enhanced by the SBR to promote hydrogen production. Rape-seed dregs are available everywhere. Therefore, it is feasible for rape-seed dregs to produce hydrogen.