篇名 | Selectively Concentrating Cervical Carcinoma Cells from Red Blood Cells Utilizing Dielectrophoresis with Circular ITO Electrodes in Stepping Electric Fields |
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卷期 | 33:1 |
作者 | Ching-Te Huang 、 Tamara G. Amstislavskaya 、 Guang-Hong Chen |
頁次 | 051-058 |
關鍵字 | Isolation 、 Dielectrophoresis 、 Stepping electric field 、 Rare cells 、 EI 、 SCI |
出刊日期 | 201302 |
The detection of rare cells, such as circulating tumor cells, circulating fetal cells, and stem cells, is important for medical diagnostics and characterization. The present study develops a handheld electric module which provides stepping electric fields for dielectrophoresis (DEP) to selectively concentrate cervical carcinoma cells (HeLa) from red blood cells, making it low-cost and automated. To observe the experiments, transparent electrodes were fabricated by patterning indium-tin-oxide-coated glass. Positive dielectrophoretic cells were guided toward the center of the microchamber due to the movement of the high-electric-field region. The magnitude of the DEP force acting on HeLa cells is about seven-fold that acting on red blood cells under a given electric field distribution, making it possible to separate HeLa cells from normal blood cells. HeLa cells were successfully concentrated in 160 seconds with an applied peak-to-peak voltage of 16 V at a frequency of 1 MHz.