The photo stimulation of skin can possibly improve wound healing. However, the effects of light stimulation on the process of wound healing are not fully understood. This study shows that red light emitted from a light-emitting diode (LED) device can effectively stimulate the proliferation of human foreskin fibroblasts (HFFs) and the synthesis of important cytokines related to wound healing. The proliferation of HFFs was found to be significantly accelerated after exposure to a red LED light (630 nm) at 16.3 mWcm-2 for 400 seconds. The syntheses of Transforming growth factor beta 1, Keratinocyte growth factor, and Adenosine triphosphate were increased by light irradiation. Importantly, our findings indicate that the LED light irradiation could stimulate the proliferation of fibroblasts and may facilitate the wound healing.