本研究目在於確認海藻萃取物是否會對 AhR、PPAR 受體產生反應。首先，藻類(螺旋藻屬，學名：Spirulina)先以乙酸乙酯進行萃取，接著配取11 個不同比例之丙酮及正己烷(v丙/v正: 10/0、9/1、8/2、7/3、6/4、5/5、4/6、3/7、2/8、1/9、0/10)混合液進行淨化，再進行溶劑轉換，溶於二甲亞颯 (Dimethyl sulfoxide, DMSO)中。本實驗使用兩種人類癌細胞株Huh7-PPRE-Luc 以及Huh7-DRE-Luc，探討藻類萃取液與三種內分泌干擾物DEHP、BPA、DIOXIN 是否對PPAR、AhR 等機制產生影響。結果發現3/7(丙酮/正己烷)層級時含有可誘導Huh7-DRE-Luc 細胞株反應之成份，9/1 與10/0 層級則可誘導Huh7-PPRE-Luc 細胞株反應。將藻類萃取液與10 nM 2,3,7,8-TCDD 共處理後，在3/7 層級可誘導247%冷光反應值，有加成作用情形產生，但分析結果與其他10 個層級相比未達顯著差異。萃取液於9/1 層級可誘導Huh7-PPRE-Luc 冷光讀值反應，與10 nM DEHP 標準品共處理後，則產生抑制的現象。藻類萃取液與10 nM BPA 標準品共處理後，同樣在9/1 與10/0 兩個層級出現抑制的現象，與DEHP具有相同的影響。DEHP 與BPA 化合物可能會誘發人體的PPAR 反應，但某些萃取物可以抑制DEHP 與BPA 冷光反應。

The aim of the present study was to determine whether the algae extract mediated the responsesof nuclear receptors with AhR and PPAR. Algae (Genus: Spirulina) was extracted by acetic acetateand the extract was passed through the silica-gel column. Eleven fractionated samples werecollected by thin layer chromatograph with 11 mixture of acetone and n-hexane (va/vh: 10/0, 9/1, 8/2,7/3, 6/4, 5/5, 4/6, 3/7, 2/8, 1/9, and 0/10), and then, they were dissolved in dimethyl sulfoxide(DMSO) after exchanging with solvent. Two human recombinant hepatoma cells (Huh7-PPRE-Luccells and Huh7-DRE-Luc cells) and three endocrine disruptors [di-(2-ethyl hexyl) phthalate (DEHP),bisphenol-A (BPA) and 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD)] were tested in this study. Weonly found that the elute in the fraction of 3/7 (va/vh) induced AhR-luciferase activities and the elutein the fraction of 9/1 and 10/0 (va/vh) availably enhanced the responses of PPRE-luciferaseactivation. Although the algae-extract fraction of 3/7 (va/vh) increased 2,3,7,8-TCDD-mediatedluciferase activation up to 247% after cotreating with the extract and 10 nM TCDD inHuh7-DRE-Luc cells, the enhanced luciferase activation was not significantly different from thosein the other fractions. For Huh7-PPRE-Luc cells, the significantly inhibitory effects of DEHP andBPA-induced luciferase activation were found in the algae-extract fractions of 9/1 and 10/0 (va/vh),respectively, after treating with 10 nM DEHP and BPA. Parts of the fractionated elution were treatedwith 10 nM DEHP and BPA possibly to induce the responses of PPAR activation in the recombinantcells. According to our preliminary results, the algae extracts may be potential for inhibitory ofDEHP or BPA-PPRE mediated luciferase activation.