戴奧辛污染是國人極為關注之環境議題，且戴奧辛對於自然環境及人類健康之毒害日益受到重視，戴奧辛造成的毒性反應範圍相當廣泛，可分為皮膚毒性、免疫、神經、內分泌、生殖毒性與致癌性等。一般檢測戴奧辛的方法是利用高解析氣相層析質譜儀(High resolution gaschromatograph/high resolution mass spectrometer, HRGC/HRMS)來進行分析，由於此項化學分析成本過高且耗時，因此需要快速且低成本的分析方法進行大量樣品分析。在歐、美、日等已開發國家，均已開發出化學活化冷光酵素報導基因法(Chemically Activated Luciferase Expression ,CALUX)，並以該技術為戴奧辛初步篩檢方法，以降低社會檢測戴奧辛所付出的成本。本研究將先前成功開發帶有戴奧辛反應單元的人類肝癌細胞株Huh7-DRE-Luc 之生物分析法，嘗試以添加藥物(Phorbol-12-myristate-13-acetate , PMA)與降低暴露溫度至35 ℃進行測試。結果得到降低溫度與添加藥物可得到冷光數值顯著上升，並跟未加藥與降低溫度之最低工作範圍由100pM 降至50 pM，且50 pM 之後冷光數值皆比未降溫及加藥時高出5~10 倍；藉由此方法檢測16 件次土壤樣品與21 件次底泥樣品，迴歸分析結果顯示土壤與HRGC/HRMS 作其相關性R2=0.9988 ( p value<0.0001)，底泥樣品與HRGC/HRMS 相關性R2=0.9955 ( p value<0.0001)，土壤與底泥樣品之生物分析法與化學分析法平均比值分別為4.65 與3.92 倍。依據本研究結果，降溫與添加PMA 可加大Huh7-DRE-Luc 的工作範圍，並改善與化學分析法的比值。

Dioxins are recognized as endocrine disruptors in the environment. Dioxins are raising publicconcern because of their highly toxic potential associated with several adverse health effects includingskin lesions, impairment of the immune system, the delayed development of nervous system,interference with the endocrine system and reproductive functions, and cancers. A high-resolution gaschromatography coupled with a high-resolution mass spectrometry (HRGC/HRMS) is currently themost widely used method to determine dioxin concentrations. However, this method requiresexpensive equipment and highly trained analysts, whilst the sample preparation procedures are oftentime consuming and expensive. Therefore, we need a fast and low cost analytical method for thelarge-scale dioxin surveillance. In this study we developed Huh7-DRE-Luc cell lines which were suchsimilar to Chemically Activated Luciferase Expression (CALUX). Owing to enhancement ofluciferase activation, by Phorbol-12-myristate-13-acetate (PMA) was added and the culture incubationtemperature was changed from 37 to 35°C. After the changes, we lowered the minimum effectiveconcentration from 100 to 50 pM and luciferase activation was increased 5-10 times compared to theunchanged control. The TCDD-induced luciferase activation was increased in 16 soil and 21 sedimentsamples in comparison with the unchanged control. The correlation between HRGC/HRMS andHuh7-DRE-Luc in the samples of soil and sediment were R2=0.9988 (p<0.0001) and 0.9955 ( p<0.0001), respectively. The average ratios of Huh7-DRE-Luc/HRGC-HRMS in soils and sedimentswere 4.65 and 3.92, respectively. Finally, the findings of the present study showed that the addition ofPMA and lowering incubation temperature had affected the enhancement of TCDD-induced luciferaseactivation in Huh7-DRE-Luc cells.