本研究以陽極氧化鋁膜（AAO)之背阻障層結構做為模仁的3D奈米結構圖形，於AAO阻障層結構上濺鍍一層金薄膜做為電極，接著利用電鑄技術於此電極上鍍上鎳，最後將AAO蝕刻掉，即得到具有3D奈米結構之鎳模仁。本研究進一步以奈米結構之金屬模仁壓印聚（乳酸一甘醇酸共聚物）（PLGA)、聚乳酸(PLA)、幾丁聚醣（Chitosan)等生物可降解材料，製作奈米結構組織工程支架。

In this study, we demonstrate a replica mold fabrication method for nano-hemisphere arrays nanomolding by nickel electroforming using the highly ordered nano-hemisphere array of the barrier-layer surface of an anodic aluminum oxide (AAO) membrane as the master mold. The feature size of the nano-hemispheres can be controlled by the use of different etching solutions for anodic oxidation of aluminum. Using the nickel replica mold, nano-structured tissue engineering scaffolds in poly(lactic-co-glycolic acid) (PLGA), polylactide (PLA), and chitosan were fabricated by casting.