The adoption of novel technologies such as radio-frequency identification (RFID) along the healthcare value chain are transforming the sector by enabling automatic identification and tracking of products, people, and assets, resulting in real-time visibility and improved efficiency in the delivery of healthcare services. Although a lot of information on RFID applications within the healthcare sector is already available, there is a need to clarify the role of RFID-specific technologies and systems in supporting such innovative healthcare applications. This paper provides a background for healthcare practitioners and researchers to clarify the role of RFID technologies in the healthcare sector. It then proposes a typology of the main RFID-enabled application domains and identifies specific technological designs that support such innovative healthcare applications. The living laboratory approach is used to assess and validate various technological designs that can be selected when envisioning the implementation of such applications.