篇名 | 桂竹葉中黃酮成份之抗氧化活性探討 |
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卷期 | 19 |
並列篇名 | The Antioxidant Activities of Flavonoids in Phyllostachys Makinoi Hayata |
作者 | 劉柏村 、 黃俊薰 、 何睿芬 、 黃文盈 |
頁次 | 031-034 |
關鍵字 | 高效液相層析 、 黃酮 、 桂竹 、 抗氧化力 、 high performance liquid chromatography 、 flavonoid 、 Phyllostachys makinoi Hayata 、 antioxidant activity |
出刊日期 | 201312 |
DOI | 10.6192/CGUST.2013.12.19.4 |
本研究以高效液相層析儀分析桂竹之黃酮成分,利用流動相A (acetonitrile/methanol = 2:1) 與流動相B (H2O),以流速0.8 mL/min沖提50分鐘,在254 nm下可分析2個成分:vitexin與isovitexin。再以清除DPPH自由基方法測定桂竹之抗氧化力,實驗結果顯示,清除DPPH自由基能力IC5Q爲0.540 土 0.027 mg/mL,可知桂竹具有抗氧化力。
In this study, high performance liquid chromatography was used to analysis the flavonoids of Pnyllostackys makinoi Havata. Eluting with mobile phase A (acetomtnle / methanol = 2:1) and mobile phase B (H2O) at a flow rate of 0.8 mL / min for 50 minutes and 254 nm, the following four components could be analyzed: vitexin and isovitexin. Furthermore, the DPPH radical scavenging activity was examination to determine the antioxidant activity of P. makinoi Hayata. The result showed the P. makinoi Hayata had antioxidant activity and IC50 of DPPH scavenging capacity was 0.540 土 0.027 mg /mL.