本研究以高效液相層析搭配光二極體陣列檢出器，同步分析膠囊及錠劑中的β-胡蘿蔔素、葉黃素、番茄紅素與玉米黃素。檢體先經水分散後，再以乙酸乙酯進行萃取，並以高效液相層析儀進行分析。使用之層析管柱為YMC Carotenoids C30 (4.6 × 250 mm，粒徑5 μm)，移動相溶液為含有0.05%三乙胺的乙腈溶液及0.05%三乙胺的甲基三級丁基醚溶液梯度沖提，流速為1 mL/min；β-胡蘿蔔素、葉黃素及玉米黃素之檢測波長為450 nm，番茄紅素為470 nm。經確效後，此方法之線性範圍：β-胡蘿蔔素0.1-2 μg/mL、葉黃素0.05-1 μg/mL、番茄紅素5-100 μg/mL及玉米黃素0.5-10 μg/mL，線性回歸方程式之判定係數 (R2) 介於0.9960-0.9980。分別添加此四種標的成分標準品於類似樣品基質中，檢測後回收率為92.3-112.2%；重複性及中間精密度之變異係數分別為0.17-4.74%及0.13-5.72%。β-胡蘿蔔素、葉黃素、番茄紅素及玉米黃素之定量極限分別為10、5、500及50 μg/g。再以此同步分析方法檢測標準參考物質SRM 3280，其檢測值均落於規格範圍內。以所建立的方法分析市售檢體四件，進一步確認方法之應用性，其結果顯示符合性為84-137%。

A high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous determination of β-carotene, lutein, lycopene and zeaxanthin in tablet and capsule products. Samples were dispersed in water and extracted with ethyl acetate before analysing with HPLC. The HPLC was performed on a YMC Carotenoids C30 (4.6 × 250 mm) column and a gradient mobile phase profile, and the flow rate was 1 mL/min. The chromatography was detected at 450 nm for β-carotene, lutein and zeaxanthin, and 470 nm for lycopene. The specificity of the method was demonstrated by the retention characteristics, HPLC spectra and by comparing the peak purity with the standard of each compound. The calibration curves (β-carotene 0.1-2 μg/mL, lutein 0.05-1 μg/mL, lycopene 5-100 μg/mL and zeaxanthin 0.5-10 μg/mL) plotted with five concentrations of each compound were linear with a determination coefficient R2 > 0.9960. It was found to be satisfactory in terms of accuracy (recovery between 92.3% and 112.2%) and precision (intra-day and inter-day variability between 0.17-4.74% and 0.13-5.72%, respectively). The limits of quantification (LOQ) were 10, 50, 500 and 50 μg/g for β-carotene, lutein, lycopene and zeaxanthin, respectively. The HPLC method was applied to SRM3280, a standard reference material for Multivitamin/Multielement Tablets, and the results all met the specification of SRM 3280. Four samples purchased from the market were analyzed by this method to ensure the applicability. The results showed that the consistency is between 84-137%.