篇名 | 廣藿香抗氧化成分之分離純化及結構鑑定 |
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卷期 | 51:3 |
並列篇名 | Separation, Isolation and Identification of Antioxidative Components from Pogostemon chablin |
作者 | 盧錫祺 、 喬長誠 、 柯宏達 、 陳昱翔 、 熊皓儀 、 蘇正德 |
頁次 | 120-126 |
關鍵字 | 廣藿香 、 抗氧化成分 、 多酚配苷 、 Pogostemon chablin 、 antioxidative component 、 polyphenol glycoside. 、 Scopus 、 TSCI |
出刊日期 | 201306 |
本研究探討廣藿香 (Pogostemon chablin) 之抗氧化成分,廣藿香粉末依序以正己烷、乙酸乙酯及甲醇萃取、過濾、濃縮後檢測其抗氧化性,三種溶劑萃取物之抗氧化性依序為BHA > 甲醇萃取物 > α-生育醇 > 乙酸乙酯萃取物 > 正己烷萃取物 > 控制組。選擇具抗氧化較佳之廣藿香甲醇萃取物,經XAD-7液相層析區分出顯現強抗氧化性的沖提部分,利用高效液相層析法分析,選擇具抗氧化性,且所含成分較不複雜之區分,重複以高效液相層析分離純化,藉各種光譜分析法鑑定,得到三種純化物質,分別為adenosine、4-hydroxyethylcatachol-1-O-(2'-α-L-rhamnopyranosyl)-β-D glucopyranoside及ο-coumaroyl-β-D-glucopyranoside。
The purpose of this study was to investigate the antioxidative components from Pogostemon chablin. The ground powder of Pogostemon chablin was sequentially extracted with n-hexane, ethyl acetate and methanol. The antioxidative activity of the extract was measured by the ferric thiocyanate method. The order of antioxidative activity was: BHA > methanol extract > α-tocopherol > ethyl acetate extract > n-hexane > control. Due to the strong antioxidative activity of methanol extract, it was fractionated and isolated by various chromatographic method to obtain three pure components identified as adenosine,4-hydroxyethylcatachol- 1-O-(2'-α-L-rhamnopyranosyl)-β-D-glucopyranoside, ο-coumaroyl-β-D-glucopyranoside.