動物檢體中之農藥殘留量可能來自污染的環境或食物鏈。為確保食物的安全及人類的健康，衛生福利部依禽畜種類及不同殘留部位訂有「禽畜產品中殘留農藥限量標準」，另我國水產品外銷日本亦曾被檢出農藥殘留，故建立快速而環保之動物檢體中農藥多重殘留分析方法有其必要性。本研究以鮭魚、豬肉和雞肉等三種肉品為測試檢體；依文獻中曾於動物檢體中監測到之品項及其物化特性 (physicochemical properties) 中挑選毆殺斯 (oxadixyl) 等26品項農藥，以QuEChERS前處理技術結合氣相層析串聯質譜儀 (gas chromatograph tandem mass spectrometer, GC/MS-MS) 進行多重殘留分析方法開發。為提升靈敏度，檢液濃縮四倍並採大體積進樣 (large volume injection, LVI) 注射模式進行分析，以基質匹配檢量線進行定量。試驗檢體分別以添加量0.002-0.03 ppm(n = 5) 進行確效試驗，26品項農藥中，二氯松 (dichlorvos)、芬化利 (fenvalerate) 和第滅寧(deltamethrin) 不適用此方法；六氯苯 (hexachlorobenzene, HCB)、滅蟻樂 (mirex) 和五氯硝基苯 (quintozene, PCNB) 則因親脂性高的特性使回收率偏低 (30-60%) 但重複性良好；其餘20品項 (地特靈 (dieldrin) 於豬肉檢體除外) 之平均回收率 (60-117%) 及重複性皆良好。本研究所開發之QuEChERS多重殘留分析方法，適用於魚、豬和雞肉等檢體中oxadixyl等23項農藥之檢驗，定量極限介於0.002-0.01 ppm之間，未來將持續擴增農藥品項及檢體類別，以作為公告方法研擬
之參考。

The pesticide can be transferred to animals by the polluted environments or the food chain system. To ensure the food safety and human health, maximum pesticide residue levels for livestock products has been set by Ministry of Health and Welfare. Some pesticide residues have been detected in our aquatic products exported to Japan. It is important to establish a quick and green method for multiple pesticide residues analysis in animal matrices. 26 pesticides were selected in this study according to their physicochemical properties and the survey results of animal products. A method was developed to determine the residues of 26 pesticides in salmon, pork and chicken by a QuEChERS method with gas chromatograph/tandem mass spectrometer (GC/MS-MS). The final extract was concentrated 4 times with large volume injection (LVI) type to increase method sensitivity. Quantification of pesticides were conducted by matrix-matched calibration curves. Samples spiked with a LOQ level 0.002-0.03 ppm (n = 5) respectively were used to validate this method. Dichlorvos, fenvalerate and deltamethrin can not be measured by this method. The recoveries of lipophilic pesticides (hexachlorobenzene, mirex and quiutozene) were lower (30-60%) but well repeatability. The remaining 20 compounds (except dieldrin in pork) had both satisfied recovery (60-117%) and well repeatability. The limits of quantitation were ranged from 0.002 to 0.01 ppm. Items of pesticide and categories of matrix will be increased in the future to validate this method. The results will be used as the references for the Taiwan official method.